High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays
High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays
复制标题
DOI：
10.1007/978-1-61779-286-1_15
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Yue, Tingting
中科院分区：
文献类型：
--
作者：
Haab, Brian B.;Yue, Tingting
抗体-凝集素夹心阵列 (ALSA) 是糖蛋白组学研究的强大新工具。 ALSA 能够精确测量直接从生物样品中捕获的多种蛋白质的糖基化状态。该平台可以以低样品消耗的高通量模式使用，使其非常适合探索特定蛋白质的聚糖改变的生物标志物研究。本文详细介绍了 ALSA 的使用，特别关注生物标志物研究。涵盖了抗体和凝集素的制备和选择、阵列和样品的制备和使用，以及使用该平台进行生物标志物研究的特殊注意事项。
The antibody-lectin sandwich arrays (ALSA) is a powerful new tool for glycoproteomics research. ALSA enables precise measurements of the glycosylation states of multiple proteins captured directly from biological samples. The platform can he used in a high-throughput mode with low sample consumption, making it well suited to biomarker research exploring glycan alterations on specific proteins. This article provides detailed descriptions of the use of ALSA, with a particular focus on biomarker research. The preparation and selection of antibodies and lectins, the preparation and use of the arrays and samples, and special considerations for using the platform for biomarker research are covered.