BINDING AND STIMULATION OF HIV-1 INTEGRASE BY A HUMAN HOMOLOG OF YEAST TRANSCRIPTION FACTOR SNF5
BINDING AND STIMULATION OF HIV-1 INTEGRASE BY A HUMAN HOMOLOG OF YEAST TRANSCRIPTION FACTOR SNF5
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DOI：
10.1126/science.7801128
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发表时间：
1994-12-23
期刊：
影响因子：
56.9
通讯作者：
GOFF, SP
中科院分区：
文献类型：
--
作者：
KALPANA, GV;MARMON, S;GOFF, SP
逆转录病毒进入宿主细胞后，会引导病毒RNA基因组的逆转录，并建立整合的前病毒DNA。逆转录病毒整合酶蛋白（IN）负责将病毒DNA插入宿主染色体靶点。利用双杂交系统鉴定出一种人类基因产物，它在体外与人类免疫缺陷病毒1型（HIV - 1）整合酶紧密结合，并刺激其DNA连接活性。该基因的序列表明，这种蛋白质是酵母SNF5的人类同源物，酵母SNF5是许多基因高水平表达所必需的转录激活因子。这个名为INI1（整合酶相互作用因子1）的基因可能编码一种核因子，该因子促进整合并将进入的病毒DNA靶向活性基因。
Upon entry into a host cell, retroviruses direct the reverse transcription of the viral RNA genome and the establishment of an integrated proviral DNA. The retroviral integrase protein (IN) is responsible for the insertion of the viral DNA into host chromosomal targets. The two-hybrid system was used to identify a human gene product that binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity. The sequence of the gene suggests that the protein is a human homolog of yeast SNF5, a transcriptional activator required for high-level expression of many genes. The gene, termed INI1 (for integrase interactor 1), may encode a nuclear factor that promotes integration and targets incoming viral DNA to active genes.