Investigation of the damage on the outermost hair surface using ToF-SIMS and XPS
Investigation of the damage on the outermost hair surface using ToF-SIMS and XPS
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DOI：
10.1002/sia.3878
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发表时间：
2012-06-01
影响因子：
1.7
通讯作者：
Aoyagi, Satoka
中科院分区：
文献类型：
--
作者：
Okamoto, Masayuki;Ishikawa, Kazutaka;Aoyagi, Satoka
人类头发的最外层表面覆盖着一层薄薄的脂肪酸结合蛋白，它在赋予头发柔软光滑的感觉和表面疏水性方面起着重要作用。在这项研究中，脂肪酸和蛋白质碎片离子的变化，其特征在于使用飞行时间二次离子质谱（ToF-SIMS）和X射线光电子能谱（XPS）调查头发损伤。结果，与毛发角蛋白的主要组分胱氨酸和半胱氨酸相关的碎片离子m/z 76的强度随着毛发损伤而降低，这与脂肪酸中的硫酯键和胱氨酸的二硫键的氧化裂解引起的半胱氨酸的产生一致。因此，表明可以通过测量m/z 76来评价毛发损伤。脂肪酸的主要成分18-甲基二十烷酸在三个漂白过程中迅速减少，m/z 76处的峰也相应减少。这些结果表明，氧化断裂的硫酯键的脂肪酸是漂白过程中所造成的主要变化。版权所有（c）2011约翰威利父子有限公司
The outermost surface of the human hair is covered with a thin layer of fatty acids bonding proteins, which plays an important role in giving hair a soft and smooth feeling and surface hydrophobicity. In this study, changes in fatty acids and protein fragment ions were characterized using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) to investigate hair damage. As a result, the intensity of the fragment ion m/z 76 related to cystine and cysteine, major components of hair keratin, decreased with the hair damage, which is consistent with generation of cysteic acid caused by oxidative cleavage of both thioester bond in the fatty acids and disulfide bond of cystine. Thus, it is indicated that hair damage can be evaluated by measuring m/z 76. Furthermore, 18-methyleicosanoic acid, the major component of the fatty acids, decreased rapidly in three bleaching processes, and the peak at m/z 76 also decreased correspondingly. These results suggest that the oxidative cleavage of thioester bonds in the fatty acids is the major change caused by the bleaching process. Copyright (c) 2011 John Wiley & Sons, Ltd.