Association of tobacco etch virus related RNA with chloroplasts in extracts of infected plants.
Association of tobacco etch virus related RNA with chloroplasts in extracts of infected plants.
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DOI:
10.1016/0042-6822(86)90292-8
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发表时间:
1986-04
期刊:
影响因子:
3.7
通讯作者:
I. P. Gadh;V. Hari
中科院分区:
文献类型:
--
作者:
I. P. Gadh;V. Hari
The RNA in various subcellular fractions of tobacco etch virus (TEV) infected tissue was analyzed for the presence of complementary viral RNA, viral RNA, and double-stranded viral RNA by hybridization with32P-labeled viral RNA or cDNA probes. Although viral RNA was detected in several cellular fractions, the complementary RNA of full-length size was found exclusively associated with fractions containing chloroplasts. Treatment of RNAs with RNase before hybridization suggested that the virus-related complementary RNA was present in double-stranded form.