Disquisitions on Original Antigenic Sin. II. Proof in lower creatures.
Disquisitions on Original Antigenic Sin. II. Proof in lower creatures.
复制标题
关于原始抗原罪的歧视。 ii。较低生物的证明。
DOI：
10.1084/jem.124.3.347
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发表时间：
1966-09-01
期刊：
影响因子：
--
通讯作者：
Webster RG
中科院分区：
文献类型：
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作者：
Fazekas de St Groth;Webster RG
在兔子身上进行的实验是为了检验在论文（1）中提出的两个未经证实的假设：原始抗原罪基本上是一种受限制的回忆反应，存在一种捕获机制，能够将抗原从一种细胞转移到另一种细胞。它表明，全身X射线照射，足以消除初级，但不是二级抗体的生产，离开原始抗原罪的所有表现不变。这证明了第一个假设。用非常大剂量的相关抗原激发的初次免疫动物产生交叉反应性抗体的立即应答，随后是对激发抗原的标准初次应答。当用两种抗原的适当混合物加强免疫时，应答是对同源抗原的标准二级应答，然后是对交叉反应抗原的标准初级应答。当动物主要接种两种相关抗原的混合物时，小剂量的任一种都只会刺激标准的二次应答。当这些动物被给予非常大剂量的任一抗原时，应答是针对相关抗原的同源次级和原始抗原Sin型的复合物。这些发现都证明了第二个假设的必然结果。结果进行了讨论的限制，他们施加的理论与生产的抗体。
Experiments in rabbits were designed to test the two unproven assumptions of the hypothesis proposed in the companion paper (1): that Original Antigenic Sin is fundamentally a restricted anamnestic response, and that there exists a trapping mechanism capable of deflecting antigen from one kind of cell and guiding it to another. It is shown that whole-body X-irradiation, sufficient to abolish primary but not secondary production of antibodies, leaves all manifestations of the Original Antigenic Sin untouched. This proves the first assumption. Primary immune animals challenged with very large doses of a related antigen produce an immediate response of cross-reactive antibodies, followed by a standard primary response to the challenging antigen. When boosted with an appropriate mixture of both antigens, the response is of standard secondary type to the homologous antigen, followed by a standard primary response to the crossreacting antigen. When animals are primarily vaccinated with a mixture of two related antigens, small booster doses of either will stimulate a standard secondary response only. When such animals are given very large booster doses of either antigen, the response is a compound of a homologous secondary and of an Original Antigenic Sin-type against the related antigen. Each of these findings demonstrates a corollary of the second assumption. The results are discussed in terms of the limitations they impose on theories concerned with the production of antibodies.