Hernocompatibility evaluation of poly(glycerol-sebacate) in vitro for vascular tissue engineering
Hernocompatibility evaluation of poly(glycerol-sebacate) in vitro for vascular tissue engineering
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DOI：
10.1016/j.biomaterials.2006.04.010
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发表时间：
2006-08-01
期刊：
影响因子：
14
通讯作者：
Ameer, Guillermo A.
中科院分区：
文献类型：
--
作者：
Motlagh, Delara;Yang, Jian;Ameer, Guillermo A.
聚（甘油-癸二酸酯）（PGS）是一种弹性可生物降解的聚酯，可能用于体内血管工程。然而，其血液材料的相互作用是未知的。本研究的目的是：（a）制造基于PGS的双相管状支架和（B）在体外评估PGS的血液相容性，以了解其在体内的潜在用途。通过用PGS预聚物浸涂玻璃棒将PGS掺入到双相支架中。使用新鲜人血和人单核细胞系（THP-1）评价PGS的促凝性（血小板粘附和聚集）和炎症潜力（IL-1 β和TNF α表达）。通过测量THP-1细胞上的组织因子表达、血浆凝固时间和全血凝固时间来评估凝血系统的活化。使用玻璃、组织培养塑料（TCP）、聚（l-丙交酯-共-乙交酯）（PLGA）和膨胀型聚四氟乙烯（ePTFE）作为参照材料。成功制备了以PGS为血液接触表面的双相支架。相对于玻璃（100%），ePTFE、PLGA和PGS上的血小板附着分别为61%、100%和28%。与ePTFE和PLGA相比，PGS引起THP-1细胞释放的IL-1 β和TNF α显著更低。类似地，相对于所有参考材料，当暴露于PGS时，THP-1细胞的组织因子表达降低。PGS的血浆凝固和全血凝固特征与测试的参考聚合物的血浆凝固和全血凝固特征相当或更好。(c)2006爱思唯尔有限公司保留所有权利。
Poly(glycerol-sebacate) (PGS) is an elastomeric biodegradable polyester that could potentially be used to engineer blood vessels in vivo. However, its blood-material interactions are unknown. The objectives of this study were to: (a) fabricate PGS-based biphasic tubular scaffolds and (b) assess the blood compatibility of PGS in vitro in order to get some insight into its potential use in vivo. PGS was incorporated into biphasic scaffolds by dip-coating glass rods with PGS pre-polymer. The thrombogenicity (platelet adhesion and aggregation) and inflammatory potential (IL-1 beta and TNF alpha expression) of PGS were evaluated using fresh human blood and a human monocyte cell line (THP-1). The activation of the clotting system was assessed via measurement of tissue factor expression on THP-1 cells, plasma recalcification times, and whole blood clotting times. Glass, tissue culture plastic (TCP), poly(l-lactide-co-glycolide) (PLGA), and expanded polytetrafluorethylene (ePTFE) were used as reference materials. Biphasic scaffolds with PGS as the blood-contacting surface were successfully fabricated. Relative to glass (100%), platelet attachment on ePTFE, PLGA and PGS was 61%, 100%, and 28%, respectively. PGS elicited a significantly lower release of IL-1 beta and TNF alpha from THP-1 cells than ePTFE and PLGA. Similarly, relative to all reference materials, tissue factor expression by THP-1 cells was decreased when exposed to PGS. Plasma recalcification and whole blood clotting profiles of PGS were comparable to or better than those of the reference polymers tested. (c) 2006 Elsevier Ltd. All rights reserved.