Low and homogeneous copy number of plasmid-borne symbiont genes affecting host nutrition in Buchnera aphidicola of the aphid Uroleucon ambrosiae
Low and homogeneous copy number of plasmid-borne symbiont genes affecting host nutrition in Buchnera aphidicola of the aphid Uroleucon ambrosiae
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DOI：
10.1046/j.1365-294x.2003.01782.x
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发表时间：
2003-04-01
影响因子：
4.9
通讯作者：
Moran, NA
中科院分区：
文献类型：
--
作者：
Plague, GR;Dale, C;Moran, NA
棉铃虫(Buchnera Aphidicola)的细菌内共生体通常为寄主提供氨基酸。亮氨酸(LeuABCD)和色氨酸(TrpEG)生物合成基因的质粒扩增可能是一些Buchnera过度生产这些营养物质的机制。我们使用定量聚合酶链式反应来评估美洲斑潜蝇(Uroleucon Ambrosiae)中的leuABCD/trpEG拷贝变异性。LeuABCD和trpEG的丰度都是：(I)15个种群的蚜虫相似，(Ii)与其他种类的Buchnera相比低(特别是trpEG)。因此，trpEG与Buchnera的S染色体多倍体相结合的质粒定位可能在功能上限制而不是增加茶菜体内色氨酸的合成。
The bacterial endosymbiont of aphids, Buchnera aphidicola , often provides amino acids to its hosts. Plasmid amplification of leucine (leuABCD ) and tryptophan (trpEG ) biosynthesis genes may be a mechanism by which some Buchnera over-produce these nutrients. We used quantitative polymerase chain reaction to assess the leuABCD /trpEG copy variability within Uroleucon ambrosiae , an aphid with a wide diet breadth and range. Both leuABCD and trpEG abundances are: (i) similar for aphids across 15 populations, and (ii) low compared to Buchnera from other aphid species (particularly trpEG ). Consequently, the plasmid location of trpEG combined with Buchnera 's chromosomal polyploidy may functionally limit, rather than increase, tryptophan production within Uroleucon ambrosiae .