GABAergic responses of mammalian ependymal cells in the central canal neurogenic niche of the postnatal spinal cord.
GABAergic responses of mammalian ependymal cells in the central canal neurogenic niche of the postnatal spinal cord.
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DOI：
10.1016/j.neulet.2013.07.007
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发表时间：
2013-10-11
影响因子：
2.5
通讯作者：
Deuchars SA
中科院分区：
文献类型：
--
作者：
Corns LF;Deuchars J;Deuchars SA
广泛的染料偶联发生在哺乳动物脊髓室管膜细胞之间。GABA使所有脊髓室管膜细胞去极化。GABA效应由GABAA受体介导，而非GABA摄取转运体。哺乳动物出生后脊髓中央管周围的区域是一个高度可塑性的区域，与其他出生后神经源性壁龛（如脑室下区）有许多相似之处。在这个区域内，室管膜细胞已被确定为神经干细胞，但对它们的特性以及包括神经递质在内的局部环境如何影响它们知之甚少。神经递质GABA存在于中枢椎管周围，并且已知会影响其他出生后神经源性壁龛内的细胞。本研究采用全细胞膜片钳电生理学和细胞内染料负载体外Wistar大鼠脊髓切片来表征室管膜细胞及其对GABA的反应能力。室管膜细胞被定义为被动响应特性和低输入电阻。室管膜细胞间存在广泛的染料偶联；这被证实是使用间隙连接阻滞剂18β-甘草次酸的间隙连接偶联，显著增加室管膜细胞的输入阻力。GABA使所有室管膜细胞去极化；双库兰和加巴嗪对这种反应的部分拮抗表明GABAA受体参与了这种反应。巴氯芬缺乏作用表明GABAB受体不参与gaba能反应。室管膜细胞对GABA的反应能力表明，GABA可能能够影响出生后脊髓神经源性生态位内细胞的增殖和分化。
Extensive dye-coupling occurs between mammalian spinal cord ependymal cells. GABA depolarised all spinal cord ependymal cells tested. GABA effects were mediated by GABAA receptors but not GABA uptake transporters. The area surrounding the central canal of the postnatal mammalian spinal cord is a highly plastic region that exhibits many similarities to other postnatal neurogenic niches, such as the subventricular zone. Within this region, ependymal cells have been identified as neural stem cells however very little is known about their properties and how the local environment, including neurotransmitters, is capable of affecting them. The neurotransmitter GABA is present around the central canal and is known to affect cells within other postnatal neurogenic niches. This study used whole cell patch clamp electrophysiology and intracellular dye-loading in in vitro Wistar rat spinal cord slices to characterise ependymal cells and their ability to respond to GABA. Ependymal cells were defined by their passive response properties and low input resistances. Extensive dye-coupling was observed between ependymal cells; this was confirmed as gap junction coupling using the gap junction blocker, 18β-glycyrrhetinic acid, which significantly increased the input resistance of ependymal cells. GABA depolarised all ependymal cells tested; the partial antagonism of this response by bicuculline and gabazine indicates that GABAA receptors contribute to this response. A lack of effect by baclofen suggests that GABAB receptors do not contribute to the GABAergic response. The ability of ependymal cells to respond to GABA suggests that GABA could be capable of influencing the proliferation and differentiation of cells within the neurogenic niche of the postnatal spinal cord.
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影响因子：
3.1
作者：
Chapman, R. J.;Lall, V. K.;Maxeiner, S.;Willecke, K.;Deuchars, J.;King, A. E.
通讯作者：
King, A. E.
影响因子：
25
作者：
Liu, XX;Wang, Q;Bordey, A
通讯作者：
Bordey, A
脊髓损伤揭示了室室室细胞的多链分化。
影响因子：
9.8
作者：
Meletis, Konstantinos;Barnabe-Heider, Fanie;Carlen, Marie;Evergren, Emma;Tomilin, Nikolay;Shupliakov, Oleg;Frisen, Jonas
通讯作者：
Frisen, Jonas
影响因子：
3.4
作者：
Heyman, Nathanael S.;Burt, Janis M.
通讯作者：
Burt, Janis M.
DOI：
10.1016/s0736-5748(01)00056-9
发表时间：
2002-02-01
影响因子：
1.8
作者：
Leung, DSY;Unsicker, K;Reuss, B
通讯作者：
Reuss, B