Computational methods for the comparative quantification of proteins in label-free LCn-MS experiments
Computational methods for the comparative quantification of proteins in label-free LCn-MS experiments
复制标题
DOI：
10.1093/bib/bbm046
复制
发表时间：
2008-03-01
影响因子：
9.5
通讯作者：
Cagney, Gerard
中科院分区：
文献类型：
--
作者：
Wong, Jason W. H.;Sullivan, Matthew J.;Cagney, Gerard
Liquid chromatography (LC) coupled to electrospray mass spectrometry (MS) is well established in high-throughput proteomics. The technology enables rapid identification of large numbers of proteins in a relatively short time. Comparative quantification of identified proteins from different samples is often regarded as the next step in proteomics experiments enabling the comparison of protein expression in different proteomes. Differential labeling of samples using stable isotope incorporation or conjugation is commonly used to compare protein levels between samples but these procedures are difficult to carry out in the laboratory and for large numbers of samples. Recently, comparative quantification of label-free LCn-MS proteomics data has emerged as an alternative approach. In this review, we discuss different computational approaches for extracting comparative quantitative information from label-free LCn-MS proteomics data. The procedure for computationally recovering the quantitative information is described. Furthermore, statistical tests used to evaluate the relevance of results will also be discussed.
