Periodontal tissue regeneration using the cytokine cocktail mimicking secretomes in the conditioned media from human mesenchymal stem cells
Periodontal tissue regeneration using the cytokine cocktail mimicking secretomes in the conditioned media from human mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2017.01.065
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发表时间：
2017-02-26
影响因子：
3.1
通讯作者：
Hibi, Hideharu
中科院分区：
文献类型：
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作者：
Sakaguchi, Kohei;Katagiri, Wataru;Hibi, Hideharu
分泌组在条件培养基从人类间充质干细胞（MSC-CM）先前被证明，以促进牙周组织再生。将MSC-CM中的胰岛素样生长因子-1、血管内皮生长因子-A和转化生长因子-β 1混合，制成模拟MSC-CM的细胞因子混合物（cytokine cocktail，CC），并评价其对牙周组织再生的作用。CC在体外可促进狗骨髓干细胞和牙周膜细胞的迁移，以及人脐静脉内皮细胞的管形成。在体内，II类分叉缺损手术创建在狗的前磨牙。在4周的乙烯基聚硅氧烷诱导的炎症后，用或不用混合在羟丙基纤维素或釉质基质衍生物（EMD）中的CC填充缺损。8周后，牙周组织进行组织学和免疫组化评价。CC对新生骨和牙骨质的形成有促进作用。CC的成骨能力强于EMD，成牙骨质能力与EMD相当。CC可能是有希望的牙周组织再生。(C)2017爱思唯尔公司All rights reserved.
Secretomes in the conditioned media from human mesenchymal stem cells (MSC-CM) were previously demonstrated to promote periodontal tissue regeneration. By mixing insulin-like growth factor-1, vascular endothelial growth factor-A, and transforming growth factor-beta 1 which were included in MSC CM, we made the cytokine cocktail (CC) mimicking MSC-CM, and then evaluated its efficacy on periodontal tissue regeneration. In vitro, CC promoted the migration of dog bone marrow-derived stem cells and periodontal ligament cells, and the tube formation of human umbilical vein endothelial cells. In vivo, class II furcation defects were surgically created at premolars in dogs. After 4 weeks of vinylpolysiloxane-induced inflammation, defects were filled with or without CC mixed in hydroxypropyl cellulose, or enamel matrix derivative (EMD). After 8 weeks, periodontal tissues were evaluated histologically and immunohistochemically. CC showed promotional effects on angiogenesis and formation of new bone and cementum. Osteogenesis by CC was greater than that by EMD and cementogenesis by CC was as well as that by EMD. CC may be promising for periodontal tissue regeneration. (C) 2017 Elsevier Inc. All rights reserved.