FERTILIZATION BY SPERM INJECTION IN THE RABBIT
FERTILIZATION BY SPERM INJECTION IN THE RABBIT
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DOI：
10.1002/mrd.1120220107
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
KEEFER, CL
中科院分区：
文献类型：
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作者：
KEEFER, CL
将全兔精子和分离的精子核直接显微注射到金黄地鼠和兔卵的卵质内。这些注入的精子在随后的体外培养中解聚并形成男性原核。注射完整精子和精核，与注射未获能的附睾精核或射出的精子核相比，注入含有男性原核的激活的兔卵的数量显著更多(P<；0.01)。经精子注射受精的兔卵在培养22h内可以进行正常的卵裂分裂。
Whole rabbit spermatozoa and isolated sperm nuclei were microinjected directly into the ooplasm of hamster and rabbit ova. These injected sperm decondensed and formed male pronuclei during subsequent in-vitro culture. Injection of whole spermatozoa and sperm nuclei prepared by a protocol known to allow in-vitro capacitation of ejaculated spermatozoa yielded a significantly higher (P < 0.01) number of activated rabbit ova containing male pronuclei than did injection of uncapacitated epididymal sperm nuclei or ejaculated sperm nuclei. Rabbit ova fertilized by sperm injection were capable of undergoing normal-appearing cleavage division during 22 h of culture.