Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use
Heme Determination and Quantification Methods and Their Suitability for Practical Applications and Everyday Use
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DOI：
10.1021/acs.analchem.0c00415
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发表时间：
2020-07-21
影响因子：
7.4
通讯作者：
Imhof, Diana
中科院分区：
文献类型：
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作者：
Hopp, Marie-T;Schmalohr, Benjamin F.;Imhof, Diana
许多研究机构、临床诊断实验室和血库都在拼命寻找在不同的生理和病理环境以及各种研究应用中识别和量化血红素的可能性。其原因是血红素的毒性以及它作为溶血和促炎分子的事实。血红素只有在不与血红素蛋白结合时才发挥这些严重和不希望的作用。在从血红素蛋白释放后，它进入生物可利用状态（不稳定血红素），其中它与蛋白质、脂质、核酸或其他分子松散地结合。虽然目前用于血红素定量测定的方法和程序已在不同环境中使用多年，但其价值受到血红素具有挑战性的化学性质的限制。定量不足的一个主要原因是不稳定的和永久结合的血红素的分离及其高聚集潜力。因此，目前没有一种方法被用作普遍适用的标准化方法。本专题的目的是描述和总结血红素定量测定的最常见和最常用的化学、分析和生物化学方法。基于这一概述，最有前途的方法，为未来的解决方案，血红素定量突出。
Many research institutions, clinical diagnostic laboratories, and blood banks are desperately searching for a possibility to identify and quantify heme in different physiological and pathological settings as well as various research applications. The reasons for this are the toxicity of the heme and the fact that it acts as a hemolytic and pro-inflammatory molecule. Heme only exerts these severe and undesired effects when it is not incorporated in hemoproteins. Upon release from the hemoproteins, it enters a biologically available state (labile heme), in which it is loosely associated with proteins, lipids, nucleic acids, or other molecules. While the current methods and procedures for quantitative determination of heme have been used for many years in different settings, their value is limited by the challenging chemical properties of heme. A major cause of inadequate quantification is the separation of labile and permanently bound heme and its high aggregation potential. Thus, none of the current methods are utilized as a generally applicable, standardized approach. The aim of this Feature is to describe and summarize the most common and frequently used chemical, analytical, and biochemical methods for the quantitative determination of heme. Based on this overview, the most promising approaches for future solutions to heme quantification are highlighted.