A morphometrical study of the exocrine pancreatic cell in fasted and fed frogs.
A morphometrical study of the exocrine pancreatic cell in fasted and fed frogs.
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DOI：
10.1083/jcb.80.3.692
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发表时间：
1979-03
期刊：
影响因子：
--
通讯作者：
Geuze JJ
中科院分区：
文献类型：
--
作者：
Slot JW;Geuze JJ
应用形态计量学方法研究了摄食对蛙胰腺外分泌细胞超微结构的影响。测量各种细胞结构的体积和表面，并表示为每单位细胞体积。平均细胞大小不受喂养的影响。虽然蛋白质合成改变5至10倍（货车Venrooij，W. J.，和c.波特1971.生物化学生物Biophys. Acta. 247：468 - 470），在构成粗面内质网（RER）和代表总细胞膜的主要部分的膜的量方面没有观察到显著差异。RER的外观发生了变化。当禁食时，它的大部分膜排列成一堆堆紧密排列的狭窄脑池。摄食后4h内，这些池被分离并不规则扩张，核糖体在其表面变得有序，呈典型的棒状。高尔基体系统的总体积增加了两倍后，喂养。泡状和管状元件在高尔基体周边没有变化，但体积的高尔基体池和冷凝液泡分别增加了2.5倍和6倍。这些结构中存在的膜的量仅增加了1.6倍和3.5倍，这反映了进食后池的外观更加扩张和冷凝泡的圆形。喂食使每个细胞分泌颗粒的数量减半，并且胞吐的迹象比禁食动物更常见。这些研究结果表明，在青蛙胰腺细胞中，分泌蛋白的生产波动并不伴随着重要的细胞膜的破裂和更新。这可能有利于细胞对进食的快速和强烈反应。
The influence of feeding on the ultrastruct of the frog exocrine pancreatic cell was studied by morphometrical procedures. Volume and surface of various cell structures were measured and expressed per unit cell volume. The average cellular size was not influenced by feeding. Though protein synthesis changes 5-to 10-fold (van Venrooij, W. J., and C. Poort. 1971. Biochim. Biophys. Acta. 247:468-470), no significant differences were observed in the amount of membrane that constitutes the rough endoplasmic reticulum (RER) and that represented the major part of total cellular membranes. The appearance of the RER changed. When fasted, most of its membrane was arranged in stacks of tightly packed, narrow cisternae. Within 4 h after feeding, these cisternae were separated and irregularly dilated, and ribosomes became ordered in typical rosettes on their surface. The total volume of the Golgi system increased twofold after feeding. The vesicular and tubular elements at the Golgi periphery did not change, but the volumes of the Golgi cisternae and the condensing vacuoles increased 2.5- and 6-fold, respectively. The increased in the amount of membrane present in these structures was only 1.6- and 3.5-fold, which reflects the more distended appearance of the cisternae and the rounded shape of the condensing vacuoles after feeding. Feeding halved the number of secretory granules per cell, and signs of exocytosis were more common than in fasted animals. These findings suggest that, in the frog pancreatic cell, fluctuations in the production of secretory proteins are not accompanied by an important breakdown and renewal of cellular membranes. This may favor a rapid and strong response of the cell to feeding.