Evaluation of the humoral immune response to the cytolethal distending toxin of Aggregatibacter actinomycetemcomitans Y4 in subjects with localized aggressive periodontitis.
Evaluation of the humoral immune response to the cytolethal distending toxin of Aggregatibacter actinomycetemcomitans Y4 in subjects with localized aggressive periodontitis.
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DOI：
10.1111/j.1399-302x.2008.00483.x
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发表时间：
2009-04
影响因子：
--
通讯作者：
Korostoff J
中科院分区：
文献类型：
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作者：
Xynogala I;Volgina A;DiRienzo JM;Korostoff J
细胞致死膨胀毒素 (Cdt) 可能是伴放线聚集杆菌的几种毒力因子之一，伴放线聚集杆菌是局部侵袭性牙周炎 (LAP) 的主要病原体。关于人类 Cdt 特异性抗体反应知之甚少。目前的研究是对一组 LAP 患者以及年龄、种族和性别匹配的对照人群中毒素特异性抗体反应的定量和定性评估。 95 名受试者总共提供了 692 份血清样本。通过酶联免疫吸附测定对血清进行分析，以确定针对完整 Cdt 全毒素以及单个亚基蛋白（CdtA、CdtB 和 CdtC）的抗体滴度。使用中国仓鼠卵巢细胞，通过改良的集落形成试验评估了 Cdt 介导的生长抑制的中和作用。 95 名受试者中有 14 名表现出显着的血清 Cdt 结合活性。对照组和 LAP 组之间血清阳性个体的百分比或平均抗体滴度没有差异。在所有阳性样品中检测到针对三个 Cdt 亚基蛋白中每一个的结合活性。在所有血清阳性受试者的样本中检测到 Cdt 介导的生长抑制的中和（范围 20-75%）。 Cdt 是最近发现的一种放线放线菌毒力因子，能够诱导中和抗体反应，表明该毒素是在人类自然感染过程中产生的。绝大多数（23 人中的 20 人）LAP 受试者未能产生显着的抗 Cdt 反应，这可能部分解释了他们对该疾病的相对易感性。
Cytolethal distending toxin (Cdt) is potentially one of several virulence factors of Aggregatibacter actinomycetemcomitans, the prime etiological agent of localized aggressive periodontitis (LAP). Little is known regarding the Cdt-specific antibody response in humans. The current study is a quantitative and qualitative evaluation of the toxin-specific antibody response in a cohort of LAP patients and age-, race- and sex-matched controls. Ninety-five subjects provided a total of 692 serum samples. Sera were analysed by enzyme-linked immunosorbent assays to determine the titers of antibody against the intact Cdt holotoxin as well as the individual subunit proteins (CdtA, CdtB, and CdtC). Neutralization of growth inhibition mediated by Cdt was evaluated in a modified colony-forming assay using Chinese hamster ovary cells. Fourteen of the 95 subjects exhibited significant serum Cdt-binding activity. There were no differences in the percentages of seropositive individuals or in the mean antibody titers between the control and LAP groups. Binding activity was detected against each of the three Cdt subunit proteins in all of the positive samples. Neutralization of Cdt-mediated growth inhibition was detected in samples from all of the seropositive subjects (range 20–75%). Cdt, a recently identified A. actinomycetemcomitans virulence factor, is capable of inducing a neutralizing antibody response indicating that the toxin is produced during natural infection of humans. The failure of a vast majority (20 of 23) of the LAP subjects to mount a significant anti-Cdt response may in part explain their relative susceptibility to the disease.