A proteomics method revealing disease-related proteins in livers of hepatitis-infected mouse model
A proteomics method revealing disease-related proteins in livers of hepatitis-infected mouse model
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DOI：
10.1021/pr070094c
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Imai, Kazuhiro
中科院分区：
文献类型：
--
作者：
Ichibangase, Tomoko;Moriya, Kyoji;Imai, Kazuhiro
在这个后基因组时代，需要一种灵敏的定量方法对临床蛋白质组进行差异分析，以了解疾病的进展。在此，我们采用FD-LC-MS/MS方法，包括荧光衍生化（FD）、液相色谱（LC）分离和LC-串联质谱（MS/MS）鉴定，揭示了三个发育阶段转基因（Tg）和非转基因（NTg）小鼠肝脏中与肝癌发生相关的疾病相关蛋白。 6个月后，凋亡相关蛋白的表达受到抑制。 12 个月后，与呼吸、电子传递系统和抗氧化相关的蛋白质显着上调。 16 个月后，与防御、β-氧化和细胞凋亡相关的蛋白质显着受到抑制。蛋白质的这种波动表达可以解释肝癌发生的进展。该方法由于其高分辨率、灵敏度和重现性而可用于临床蛋白质组学分析。
In this post-genome era, a sensitive quantitative method is required for differential profiling analyses of clinical proteomes to understand the disease progress. Here, we adopt the FD-LC-MS/MS method, consisting of fluorogenic derivatization (FD), separation by liquid chromatography (LC), and identification by LC-tandem mass spectrometry (MS/MS), to reveal disease-related proteins in livers of hepatocarcinogenesis in transgenic (Tg) and non-transgenic (NTg) mice at three developmental stages. After 6 months, the expression of apoptosis-related proteins is suppressed. After 12 months, proteins related to respiration, the electron-transfer system, and anti-oxidation are significantly up-regulated. After 16 months, proteins related to defense, beta-oxidation, and apoptosis are significantly suppressed. This fluctuating expression of proteins could explain the progression of hepatocarcinogenesis. The method would be useful for clinical proteomics analysis because of its high resolution, sensitivity, and reproducibility.