Transgenic tobacco plants expressing the geminivirus BL1 protein exhibit symptoms of viral disease.
Transgenic tobacco plants expressing the geminivirus BL1 protein exhibit symptoms of viral disease.
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DOI：
10.1105/tpc.5.7.795
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发表时间：
1993-07
期刊：
影响因子：
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通讯作者：
Erica Pascal;Paige E. Goodlove;Leeju C. Wu;S. Lazarowitz
中科院分区：
文献类型：
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作者：
Erica Pascal;Paige E. Goodlove;Leeju C. Wu;S. Lazarowitz
两部分双生病毒，如南瓜曲叶病毒(SqLCV)，编码两个运动蛋白(MPS)，BR1和BL1，对病毒在寄主植物中的移动和随后的侵染是必不可少的。为了阐明这些MPS的生化功能并确定它们在病毒感染中的各自作用，我们获得了表达SqLCV BR1和BL1的转基因本氏烟草植株。表达BR1或截短BL1的转基因植株与野生型N.benthamiana没有明显的区别。相反，表达全长BL1的转基因植株，无论是单独表达还是与BR1结合，在生长特性和表型外观上都显著异常，叶片呈花叶状和卷曲状，因此与该寄主的典型SqLCV病症状相似。BL1定位于细胞壁和质膜部分，而BR1主要定位于微体膜部分。这些发现表明，BL1在转基因植物中的表达足以产生病毒病症状，并进一步表明BL1和BR1在病毒运动中具有不同和独立的功能。
Bipartite geminiviruses, such as squash leaf curl virus (SqLCV), encode two movement proteins (MPs), BR1 and BL1, that are essential for viral movement in and subsequent infection of the host plant. To elucidate the biochemical functions of these MPs and define their respective contributions to viral infection, we have generated transgenic Nicotiana benthamiana plants expressing SqLCV BR1 and BL1. Transgenic plants expressing BR1 or a truncated BL1 were phenotypically indistinguishable from wild-type N. benthamiana. In contrast, transgenic plants expressing full-length BL1, alone or in combination with BR1, were strikingly abnormal both in their growth properties and phenotypic appearance, with leaves that were mosaic and curled under, thus mimicking typical SqLCV disease symptoms in this host. BL1 was localized to the cell wall and plasma membrane fractions, whereas BR1 was predominantly in the microsomal membrane fraction. These findings demonstrate that expression of BL1 in transgenic plants is sufficient to produce viral disease symptoms, and they further suggest that BL1 and BR1 carry out distinct and independent functions in viral movement.