In vivo and in vitro research on the biological effects of deuterium-depleted water: 1. Influence of deuterium-depleted water on cultured cell growth.
In vivo and in vitro research on the biological effects of deuterium-depleted water: 1. Influence of deuterium-depleted water on cultured cell growth.
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发表时间：
2004
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通讯作者：
W. Bild;V. Năstasă;I. Haulica
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作者：
W. Bild;V. Năstasă;I. Haulica
贫氘水（DDW）是一种降低培养细胞环境中氘浓度的新方法。几种类型的建立的细胞系，正常和肿瘤生长在培养基中溶解DDW和比较相同的菌株，在相同的量，生长在培养基中溶解与正常的蒸馏水。在相同条件下，在用增殖触发物如细菌脂多糖（LPS）和伴刀豆球蛋白A（ConA）刺激下，使幼稚小鼠脾细胞生长。MTT法检测细胞生长增殖情况。DDW培养基中建立的细胞类型和培养的脾细胞都具有比正常培养基中培养的细胞显著更高的生长速率。在试图确定参与DDW的生长刺激的膜机制，膜质子转运蛋白Na+/H+逆向转运蛋白和H+/K+ ATP酶被其选择性阻断剂阿米洛利和兰索拉唑分别抑制。结果，但不完整，指向缺乏参与的Na+/H+反向转运蛋白和H+/K+ ATP酶的可能的影响。
Deuterium depleted-water (DDW) is a new available tool for decreasing deuterium concentration in the environment of cells in culture. Several types of established cell lines, both normal and neoplastic were grown in culture media dissolved with DDW and compared with the same strains, in the same amounts, grown in media dissolved with normal distilled water. Naive mice splenocytes were grown, under stimulation with proliferation triggers, like bacterial lipopolysaccharide (LPS) and Concanavalin A (ConA) in the same conditions. The growth and proliferation were estimated using the MTT assay. Both established cell types and explanted splenocytes in the DDW-media had a significantly higher growth rate than cell cultured in normal media. In an attempt to identify the membrane mechanisms involved in the growth stimulation by DDW, the membrane proton transporters Na+/H+ antiporter and H+/K+ATP-ase were inhibited with their selective blockers amiloride and respectively lansoprazole. The results, however incomplete, point towards a lack of involvement of the Na+/H+ antiporter and a possible implication of the H+/K+ATP-ase.