FGF-10 stimulates limb regeneration ability in Xenopus laevis
FGF-10 stimulates limb regeneration ability in Xenopus laevis
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DOI：
10.1006/dbio.2001.0180
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发表时间：
2001-05-01
影响因子：
2.7
通讯作者：
Tamura, K
中科院分区：
文献类型：
--
作者：
Yokoyama, H;Ide, H;Tamura, K
通过再生和非再生爪蟾肢体的相互移植实验，我们最近证明爪蟾肢体的再生能力取决于间充质组织，并且我们提出FGF-10可能参与这种能力（Yokoyama等人，2000年德夫219，18-29）。然而，该研究中获得的数据并不是FGF-10负责再生能力的决定性证据。因此，我们研究了FGF-10在再生能力的作用，通过直接引入FGF-10蛋白到非再生爪蟾肢树桩。外源性应用FGF-10成功刺激了再生能力，导致我们检查的所有基因表达（包括shh，msx-1和FGF-10）的再诱导和良好图案的肢体结构的再生。我们在这里首次报道了某种分子激活爪蟾肢体的再生能力，这一发现表明FGF-10可能是高等脊椎动物非再生肢体再生的关键分子。(C)北京：科学出版社.
By reciprocal transplantation experiments with regenerative and nonregenerative Xenopus limbs, we recently demonstrated that the regenerative capacity of a Xenopus limb depends on mesenchymal tissue and we suggested that fgf-10 is likely to be involved in this capacity (Yokoyama et al., 2000, Dev. Biol. 219, 18-29). However, the data obtained in that study are not conclusive evidence that FGF-10 is responsible for the regenerative capacity. We therefore investigated the role of FGF-10 in regenerative capacity by directly introducing FGF-10 protein into nonregenerative Xenopus limb stumps. Exogenously applied FGF-10 successfully stimulated the regenerative capacity, resulting in the reinduction of all gene expressions (including shh, msx-1, and fgf-10) that we examined and the regeneration of well-patterned limb structures. We report here for the first time that a certain molecule activates the regenerative capacity of Xenopus limb, and this finding suggests that FGF-10 could be a key molecule in possible regeneration of nonregenerative limbs in higher vertebrates. (C) 2001 Academic Press.