Near-Infrared Polymer Dots in the Portal-Hepatic Circulation Achieve Localization of Hepatic Carcinoma in Vivo
Near-Infrared Polymer Dots in the Portal-Hepatic Circulation Achieve Localization of Hepatic Carcinoma in Vivo
复制标题
门肝循环中的近红外聚合物点实现肝癌体内定位
DOI：
10.1021/acsabm.0c00729
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发表时间：
2020
影响因子：
4.7
通讯作者：
Xiong Liqin
中科院分区：
文献类型：
--
作者：
Zhou Di;Yang Yi Dian;Jin Long Yang;Yang Yong;Wang Shou Hua;Cai Qiang;Guan Wen Bin;Ma Fei;Xiong Liqin
本研究的目的是使用聚合物点，以探讨他们是否可以可视化二乙基亚硝胺（DENA）诱导的肝细胞癌（HCC）模型中的肿瘤病变。建立大鼠肝癌模型，分别于造模第0、30、60、90天检测血清肝功能指标和甲胎蛋白（AFP）。在对聚合物点进行表征后，将它们注射到大鼠和小鼠体内。提取大鼠的肝、脾和肾以及小鼠的胆囊，以验证聚合物点的代谢途径及其通过荧光成像对HCC和胆囊进行荧光定位的能力。分别经门静脉主干和尾静脉注射聚合物点后即刻和15 min，肝脏出现强荧光发射。一个令人满意的荧光成像效果持续了45分钟。聚合物点循环通过肝内血管内的血流，而不是细胞内的区域，可以清楚地通过使用pCLE和IVIS光谱成像系统可视化。没有荧光发射的HCC病灶的对比成像是由于肿瘤区域内缺乏正常的门肝静脉。在将聚合物点通过小鼠尾静脉注射后15分钟，也可以检测到胆囊的荧光成像。该聚合物点对肝内靶血管和肝癌病灶具有良好的荧光定位能力，在肝胆外科具有潜在的应用价值。
The present study aims to use polymer dots to explore whether they can visualize tumor lesions in a diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) model. The HCC rat model was set up, and serum liver function indexes and AFP were tested on days 0, 30, 60, and 90 of the modeling process. After characterization of the polymer dots, they were injected into the rats and mice. The liver, spleen, and kidney of rats and the gallbladder of mice were extracted to verify the metabolic pathways of the polymer dots and their capability of fluorescent localization of HCC and gallbladder by fluorescence imaging. Strong fluorescent emission from the liver appeared immediately and 15 min after the polymer dots were injected through the main portal veins and tail veins of the model rats, respectively. A satisfactory fluorescent imaging effect lasted up to 45 min. Polymer dots circulate through the bloodstream within intrahepatic vessels rather than intracellular areas and can be clearly visualized by using both the pCLE and IVIS spectrum imaging systems. Contrast imaging of HCC lesions without fluorescent emissions was due to the lack of normal portal-hepatic veins within the tumor areas. Fluorescent imaging of the gallbladder could also be detected at 15 min after the polymer dots were injected through the tail veins of mice. The polymer dots had satisfactory fluorescent localization capability for targeted intrahepatic vessels and HCC lesionsin vivoand showed potential practical value in hepato-biliary surgery.