Deconvolution of overlapping isotopic clusters improves quantification of stable isotope-labeled peptides
Deconvolution of overlapping isotopic clusters improves quantification of stable isotope-labeled peptides
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DOI：
10.1016/j.jprot.2011.04.022
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发表时间：
2011-09-06
影响因子：
3.3
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
Cappadona, Salvatore;Munoz, Javier;Heck, Albert J. R.
高分辨率质谱法和稳定同位素的使用极大地提高了我们量化蛋白质组的能力。通常，肽的相对丰度是通过鉴定同位素簇和比较肽对的峰强度来估计的。然而，当标记肽之间的质量偏移很小时，可能存在同位素簇重叠的可能性，这将妨碍定量准确性，对较重的肽具有典型的向上偏倚。在这里，我们调查了重叠峰问题对基于二甲基的定量的影响，我们确认可能需要纠正。此外，我们提出了一种基于模拟同位素分布的工具，可以在出现重叠问题时纠正重叠问题。我们证明，我们的方法导致提高准确性和精度的蛋白质定量。(C) 2011 Elsevier B.V.版权所有
High-resolution mass spectrometry and the use of stable isotopes have greatly improved our ability to quantify proteomes. Typically, the relative abundance of peptides is estimated by identifying the isotopic clusters and by comparing the peak intensities of peptide pairs. However, when the mass shift between the labeled peptides is small, there can be the possibility for overlap of the isotopic clusters which will hamper quantification accuracy with a typical upwards bias for the heavier peptide. Here, we investigated the impact of the overlapping peak issue with respect to dimethyl based quantification and we confirmed there can be need for correction. In addition, we present a tool that can correct overlapping issues when they arise which is based on modeling isotopic distributions. We demonstrate that our approach leads to improved accuracy and precision of protein quantification. (C) 2011 Elsevier B.V. All rights reserved.