Specialized techniques for site-directed mutagenesis in cyanobacteria.
Specialized techniques for site-directed mutagenesis in cyanobacteria.
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DOI：
10.1007/978-1-59745-257-1_11
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发表时间：
2007
影响因子：
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通讯作者：
E. Clerico;J. Ditty;S. Golden
中科院分区：
文献类型：
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作者：
E. Clerico;J. Ditty;S. Golden
细长聚球藻PCC 7942是研究蓝藻生物钟分子机制的理想模式系统。这种生物体的“可塑性”遗传特性促进了其染色体诱变的各种方法的发展。这些方法是基于染色体和外源DNA之间的同源重组，通过转化或接合引入蓝藻。在这里，我们描述了不同的方法来诱变染色体的S。包括插入突变、hit-and-run等位基因替换、rps 12介导的基因替换以及从异位位点、S.伸长体基因组
Synechococcus elongatusPCC 7942 is an excellent model system for studying the molecular mechanism of the circadian clock in cyanobacteria. The “plastic” genetic characteristics of this organism have facilitated the development of various methods for mutagenesis of its chromosome. These methods are based on homologous recombination between the chromosome and foreign DNA, introduced to the cyanobacteria by either transformation or conjugation. Here we describe different approaches to mutagenize the chromosome ofS. elongatus, including insertional mutagenesis, hit-and-run allele replacement,rps12-mediated gene replacement, and regulated expression of genes from ectopic sites, the neutral sites of theS. elongatusgenome.