Actinotrichia collagens and their role in fin formation
Actinotrichia collagens and their role in fin formation
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DOI：
10.1016/j.ydbio.2011.03.014
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Santos-Ruiz, L.
中科院分区：
文献类型：
--
作者：
Duran, I.;Mari-Beffa, M.;Santos-Ruiz, L.
斑马鱼鳍的骨架由鳞毛和放线毛组成。放线菌是位于每个鳞毛菌顶端的纤维，在鳍的形成中起着形态发生的作用。放线菌是由胶原蛋白与非胶原蛋白成分结合形成的。放线菌的非胶原成分(放线菌素)已被证明在鳍到肢的转变过程中起着关键作用。目前的研究集中在形成放线菌的胶原蛋白及其在鳍形成中的作用。我们发现放线菌是由I型胶原和一种由col2a1b基因编码的新型II型胶原形成的。这种II型胶原基因的第二个拷贝只在鱼类中发现，是唯一在FINS中表达的II型胶原。在放线毛菌形成细胞中均可检测到col1a1a和col2a1b。值得注意的是，他们还表达了赖氨酰羟基酶1(LH1)基因，该基因编码一种参与胶原蛋白翻译后加工的酶。在斑马鱼胚胎中的吗啉敲除实验表明，这两种胶原蛋白和LH1对于放线菌和鳍折的形态发生是必不可少的。在鳍的发育和再生过程中，Col1a1显性突变体吉娃娃在放线毛菌和鳞毛菌中都表现出异常的表型。这些证据支持放线菌由LH1翻译后加工的I型和II型胶原蛋白组成，这些胶原蛋白的正确表达和组装对于FIN的形成是必不可少的。放线菌独特的胶原蛋白组成可能在鳍骨形态发生中起作用。(C)2011 Elsevier Inc.保留所有权利。
The skeleton of zebrafish fins consists of lepidotrichia and actinotrichia. Actinotrichia are fibrils located at the tip of each lepidotrichia and play a morphogenetic role in fin formation. Actinotrichia are formed by collagens associated with non-collagen components. The non-collagen components of actinotrichia (actinodins) have been shown to play a critical role in fin to limb transition. The present study has focused on the collagens that form actinotrichia and their role in fin formation. We have found actinotrichia are formed by Collagen I plus a novel form of Collagen II, encoded by the col2a1b gene. This second copy of the collagen II gene is only found in fishes and is the only Collagen type II expressed in fins. Both col1a1a and col2a1b were found in actinotrichia forming cells. Significantly, they also expressed the lysyl hydroxylase 1 (lh1) gene, which encodes an enzyme involved in the post-translational processing of collagens. Morpholino knockdown in zebrafish embryos demonstrated that the two collagens and lh1 are essential for actinotrichia and fin fold morphogenesis. The col1a1 dominant mutant chihuahua showed aberrant phenotypes in both actinotrichia and lepidotrichia during fin development and regeneration. These pieces of evidences support that actinotrichia are composed of Collagens I and II, which are post-translationally processed by Lh1, and that the correct expression and assembling of these collagens is essential for fin formation. The unique collagen composition of actinotrichia may play a role in fin skeleton morphogenesis. (c) 2011 Elsevier Inc. All rights reserved.