Luteolytic effect of prostaglandin F2α on bovine corpus luteum depends on cell composition and contact
Luteolytic effect of prostaglandin F2α on bovine corpus luteum depends on cell composition and contact
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DOI：
10.1111/j.1439-0531.2007.00936.x
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发表时间：
2008-08-01
影响因子：
1.7
通讯作者：
Skarzynski, D. J.
中科院分区：
文献类型：
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作者：
Korzekwa, A. J.;Jaroszewski, J. J.;Skarzynski, D. J.
前列腺素F-2 α（PGF（2 α））是体内主要的黄体溶解因子，然而，其对牛黄体（CL）类固醇生成细胞的直接黄体溶解作用是有争议的，尚未完全了解。本研究的目的是阐明PGF（2 α）在不同的体内和体外条件下对牛CL的作用，并检查所有主要类型的CL细胞之间的接触是否是溶黄体PGF（2 α）作用所必需的。在实验1中，使用体内微透析系统用地诺前列素（PGF（2 α）的类似物）灌注牛CL（发情周期的第15天）0.5 h。地诺前列素引起孕酮（P4）的短期增加，随后其浓度降低（给药后6、10、12和24 h）。在实验2中，测定了PGF（2 α）对单层培养的CL类固醇生成细胞中P4积累的直接作用（周期的第15天）。PGF（2 α）孵育24小时后增加类固醇生成CL细胞中的P4积累。在实验3中，将类固醇生成、内皮CL和免疫细胞（周期的第15天）与PGF（2 α）在玻璃管中共培养24小时，并测定P4、一氧化氮（NO）和白三烯（LT）C-4的稳定代谢物的水平。尽管PGF（2 α）处理增加了同质类固醇生成CL细胞培养中的P4分泌，但观察到所有类型CL细胞共培养中P4分泌的减少。PGF（2 α）处理后，CL细胞的NO和LTC 4的分泌增加。内皮细胞和免疫细胞与类固醇生成CL细胞之间的相互作用是牛CL内溶黄体PGF（2 α）作用所必需的。我们的结果表明，细胞共培养模型，包括CL细胞的主要类型，是最接近的研究PGF（2 α）的作用在体外。
Prostaglandin F-2 alpha (PGF(2 alpha)) is a main luteolytic factor in vivo; however, its direct luteolytic influence on steroidogenic cells of bovine corpus luteum (CL) is controversial and not fully understood. The aim of the study was to clarify PGF(2 alpha) action on bovine CL in different in vivo and in vitro conditions and to examine whether the contact among all main types of CL cells is necessary for luteolytic PGF(2 alpha) action. In experiment 1, the bovine CL (day 15 of the oestrous cycle) was perfused using in vivo microdialysis system with dinoprost (an analogue of PGF(2 alpha)) for 0.5 h. Dinoprost caused a short-time increase in progesterone (P4), whose concentration decreased thereafter (at 6-, 10-, 12- and 24-h after treatment). In experiment 2, the direct effect of PGF(2 alpha) on P4 accumulation in CL steroidogenic cells cultured in monolayer (day 15 of the cycle) was determined. PGF(2 alpha) after 24 h of incubation increased P4 accumulation in steroidogenic CL cells. In experiment 3 steroidogenic, endothelial CL and immune cells (day 15 of the cycle) were incubated with PGF(2 alpha) in cocultures for 24 h in glass tubes and the levels of P4, stable metabolites of nitric oxide (NO) and leukotriene (LT) C-4 were determined. Although PGF(2 alpha) treatment increased P4 secretion in homogeneous steroidogenic CL cell culture, the decrease in P4 secretion in cocultures of all types of CL cells was observed. The secretion of NO and LTC4 increased after the treatment of PGF(2 alpha) both in pure cultures of CL cells and in cocultures. The interactions between endothelial and immune cells with steroidogenic CL cells are needed for luteolytic PGF(2 alpha) action within the bovine CL. Our results indicate that the cell coculture model, including the main types of CL cells, is the most approximate to study PGF(2 alpha) role in vitro.