Elements in the long terminal repeat of murine retroviruses enhance stable transformation by thymidine kinase gene.
Elements in the long terminal repeat of murine retroviruses enhance stable transformation by thymidine kinase gene.
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鼠逆转录病毒长末端重复中的元件增强了胸苷激酶基因的稳定转化。
DOI：
10.1093/nar/11.6.1855
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发表时间：
1983
影响因子：
14.9
通讯作者：
Verma,IM
中科院分区：
文献类型：
--
作者：
Jolly,DJ;Esty,AC;Subramani,S;Friedmann,T;Verma,IM
我们研究了小鼠逆转录病毒的长末端重复序列（lts）对疱疹病毒胸腺碱激酶（TK）基因获得稳定转染频率的影响。结果表明，LTRs的加入使TK+变子数量增加了10 ~ 20倍。将人或仓鼠细胞的染色体DNA与含有ltr序列的质粒混合并转染到LTK细胞中，也观察到5-12倍的增强。参与增强的LTR序列定位于包含串联重复序列的区域。LTR的所有其他区域没有表现出稳定的TK+转染的任何增强。增强子序列相对于TK基因的位置或方向对转化频率没有影响。这种增强效应似乎与染色体整合数量的增加或TK基因转录水平的升高无关。
We have investigated the effects of long, terminal repeats (LTRs) ot murine retroviruses on the frequency of obtaining stable transfectants by the herpes virus thytaiaine kinase (TK) gene. The results indicate that addition of LTRs enhances the number of TK+transformants by 10-20 fold. A 5-12 fold enhancement was also observed when chromosomal DNA from either numan or hamster cells was Bixed with a plasmid containing LTR–sequences anc transfected onto LTK’ cells. The LTR sequences involved in the enhancement were localizes in the region which contins tandem repeats. All other regions of the LTR did not show any enhancement of stable TK+transfectants. The location or the orientation of the enhancer sequences with respect to the TK gene did not exert any influence on the frequency of transformation. The enhancement effect does not appear to be linked to either increased numbers of chromosomal integrations or elevated levels of transcription of the TK gene.