Age-related increase in food spilling by laboratory mice may lead to significant overestimation of actual food consumption: implications for studies on dietary restriction, metabolism, and dose calculations.
Age-related increase in food spilling by laboratory mice may lead to significant overestimation of actual food consumption: implications for studies on dietary restriction, metabolism, and dose calculations.
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实验室小鼠与年龄相关的食物溢出增加可能导致对实际食物消耗的显着高估：对饮食限制、新陈代谢和剂量计算研究的影响。
DOI：
10.1093/gerona/gls009
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Saito,Hiroshi
中科院分区：
文献类型：
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作者：
Starr,MarleneE;Saito,Hiroshi
人们普遍认为，人类的食物消耗量会随着年龄的增长而下降。然而，来自小鼠的数据仍然存在争议。根据我们之前的观察，小鼠在进食时会溢出大量食物，我们假设老年小鼠食物溢出的增加掩盖了实际的食物摄入量。为了研究小鼠是否表现出与年龄相关的食物消耗量下降，我们通过测量食物容器中的食物量和喂食期间掉落到笼底的食物量来评估不同年龄的 C57BL/6 小鼠的实际食物消耗量。我们发现，老年小鼠的食物消耗量（36%±8%）明显多于年轻小鼠（18%±5%），这导致食物消耗量被高估，尤其是老年小鼠。尽管高龄小鼠的实际食物消耗量有所减少，但单位体重的食物摄入量并没有显着变化。这些发现表明，应谨慎准确量化老年动物的食物消耗量。
It is widely accepted that food consumption in humans declines with advanced age; however, data from mice remain controversial. Based on our previous observation that mice spill a considerable amount of food while eating, we hypothesized that increased food spillage in old mice masks actual food intake. To investigate whether mice exhibit age-associated declines in food consumption, we evaluated the actual food consumption of C57BL/6 mice at various ages by measuring both the amount of food in the food receptacle and the amount dropped to the cage bottom during feeding. We found that old mice dropped significantly more food (36% ± 8%) than young mice (18% ± 5%), which led to overestimations of food consumption, particularly in old mice. Although actual food consumption decreased in very old mice, food intake per body weight did not significantly change. These findings suggest that caution should be taken to accurately quantify food consumption by aged animals.