Molecular beacon-based quantitiation of epithelial tumor marker mucin 1.
Molecular beacon-based quantitiation of epithelial tumor marker mucin 1.
复制标题
DOI：
10.1016/j.bmcl.2012.08.037
复制
发表时间：
2012-10
影响因子：
2.7
通讯作者：
Seonmi Shin;Hye Yeon Nam;Eun Jeong Lee;W. Jung;S. Hah
中科院分区：
文献类型：
--
作者：
Seonmi Shin;Hye Yeon Nam;Eun Jeong Lee;W. Jung;S. Hah
粘蛋白1（Muc 1）是一种在大多数上皮细胞表面表达的糖蛋白，已被证实是诊断早期癌症的有用生物标志物。在这项研究中，我们证明了量子点（QD）-适体信标的作用，通过折叠诱导解离的DNA嵌入染料，BOBO-3，在存在的目标分子，Muc 1。嵌入的BOBO-3从QD缀合的适体的释放导致QD荧光共振能量转移（FRET）介导的BOBO-3发射的减少，从而允许无标记的Muc 1检测和定量。我们获得了Muc 1的高特异性和宽范围检测（从50 nM到20μM），这表明我们的QD-适体信标可以成为Muc 1检测的免疫检测的潜在替代品。该检测方法有望得到改进，用于大人群不同类型上皮癌的早期诊断。
Mucin 1 (Muc1) is a glycoprotein expressed on most epithelial cell surfaces, which has been confirmed as a useful biomarker for the diagnosis of early cancers. In this study, we demonstrate that a quantum dot (QD)-aptamer beacon acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3, in the presence of the target molecules, Muc1. Release of intercalated BOBO-3s from the QD-conjugated aptamers results in a decrease in QD fluorescence resonance energy transfer (FRET)-mediated BOBO-3 emission, allowing for label-free Muc1 detection and quantitation. We attain highly specific and wide-range detection (from 50nM to 20μM) of Muc1, suggesting that our QD-aptamer beacon can be a potential alternative to immuno-based assays for Muc1 detection. The detection methodology is expected to be improved for the early diagnosis of different types of epithelial cancers of large populations.