Engineering of geranylgeranyl pyrophosphate synthase levels and physiological conditions for enhanced carotenoid and astaxanthin synthesis in Xanthophyllomyces dendrorhous
Engineering of geranylgeranyl pyrophosphate synthase levels and physiological conditions for enhanced carotenoid and astaxanthin synthesis in Xanthophyllomyces dendrorhous
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DOI：
10.1007/s10529-010-0495-2
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Sandmann, Gerhard
中科院分区：
文献类型：
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作者：
Breitenbach, Juergen;Visser, Hans;Sandmann, Gerhard
担子菌是为数不多的可用于生物生产类胡萝卜素虾青素的微生物之一。从该真菌中克隆了crtE基因，用于萜类化合物途径的工程研究。其基因产物作为香叶基香叶基焦磷酸合成酶的功能已经建立。将crtE基因转化石杉，使其代谢产物从固醇途径转向类胡萝卜素的生物合成。获得的转化子具有更高水平的香叶基香叶基焦磷酸合成酶，导致包括虾青素在内的更高水平的类胡萝卜素。野生型和CrtE转化子最大类胡萝卜素合成的生理条件是黑暗的光照和摇瓶培养的额外供气。这些条件和crtE的转化具有加性效应，与最初没有光照和额外供气的野生型培养相比，虾青素的产量增加了8倍，生长4天内产量为451ug/g干重。
The basidiomycetous yeast, Xanthophyllomyces dendrorhous, is one of the very few organisms which can be used for biological production of the carotenoid astaxanthin. crtE cDNA has been cloned from this fungus for engineering of the terpenoid pathway. The function of its gene product as a geranylgeranyl pyrophosphate synthase was established. X. dendrorhous was transformed with the crtE cDNA to divert metabolite flow from the sterol pathway towards carotenoid biosynthesis. Transformants were obtained with increased levels of geranylgeranyl pyrophosphate synthase leading to higher carotenoid levels including astaxanthin. Physiological conditions for maximum carotenoid synthesis for wild type and the CrtE transformant were dim light and extra air supply of the shaking culture. These conditions and the transformation with crtE had additive effects and resulted in an 8-fold higher astaxanthin formation as compared to the initial wild type culture without illumination and extra air supply yielding 451 mu g/g dry wt within 4 days of growth.