Inhibition of allergen-IgE binding to B cells by IgG antibodies after grass pollen immunotherapy
Inhibition of allergen-IgE binding to B cells by IgG antibodies after grass pollen immunotherapy
复制标题
DOI：
10.1016/s0091-6749(03)02022-0
复制
发表时间：
2003-11-01
影响因子：
14.2
通讯作者：
Durham, SR
中科院分区：
文献类型：
--
作者：
Wachholz, PA;Soni, NK;Durham, SR
背景资料：在特应性个体中，过敏原特异性IgG抗体水平与过敏原致敏程度呈负相关。此外，过敏原特异性IgG抗体显着增加过敏原注射免疫疗法。这些观察结果导致了过敏原特异性IgG抗体可能在特应性individuals.Objective中具有保护特性的建议：我们假设在草花粉免疫治疗后，这些抗体破坏抗原呈递细胞对IgE依赖性过敏原的处理。我们已经开发了一种新的流式细胞术检测过敏原IgE结合的基础上，低，B细胞上的亲和力IgE受体，以检查从18名患者收集的血清的阻断效果，这些患者参加了为期1年的草花粉免疫治疗的双盲对照试验。在所有10名接受活性治疗的患者中，有抑制过敏原-IgE与B细胞结合的活性诱导（P = 0.02，与安慰剂受试者相比），以及随后的过敏原呈递给T细胞。这种活性与IgG和过敏原特异性，因为血清取自患者与草花粉免疫治疗，但谁也桦树花粉敏感不抑制IgE-桦树花粉过敏原结合到B celles.Conclusion：我们得出结论，过敏原特异性IgG抗体诱导免疫治疗可以破坏过敏原-IgE复合物的形成，结合到抗原呈递细胞，促进过敏原的介绍。
Background: Among atopic individuals, levels of allergen-specific IgG antibodies have been inversely associated with the degree of allergen sensitization. Additionally, allergen-specific IgG antibodies are markedly increased by allergen injection immunotherapy. These observations have led to proposals that allergen-specific IgG antibodies might have protective properties in atopic individuals.Objective: We hypothesized that after grass pollen immunotherapy, these antibodies disrupt IgE-dependent allergen processing by antigen-presenting cells.Methods: We have developed a novel flow cytometric assay based on detection of allergen-IgE binding to the low-affinity IgE receptor on B cells to examine the blocking effects of sera collected from 18 patients who participated in a double-blind, controlled trial of grass pollen immunotherapy for 1 year.Results: In all 10 patients who received active therapy, there was induction of activity that inhibited allergen-IgE binding to B cells (P = .02, vs placebo subjects), as well as subsequent allergen presentation to T cells. This activity copurified with IgG and was allergen specific, because sera taken from patients treated with grass pollen immunotherapy but who were also birch pollen sensitive did not inhibit IgE-birch pollen allergen binding to B cells.Conclusion: We conclude that allergen-specific IgG antibodies induced by immunotherapy can disrupt formation of allergen-IgE complexes that bind to antigen-presenting cells and facilitate allergen presentation.