Screening unlabeled DNA targets with randomly ordered fiber-optic gene arrays
Screening unlabeled DNA targets with randomly ordered fiber-optic gene arrays
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DOI：
10.1038/72006
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发表时间：
2000-01-01
影响因子：
46.9
通讯作者：
Walt, DR
中科院分区：
文献类型：
--
作者：
Steemers, FJ;Ferguson, JA;Walt, DR
我们已经开发了一种随机有序的光纤基因阵列，用于快速，平行检测未标记的DNA靶与表面固定的分子信标（MB），经历伴随着荧光变化的构象变化，在互补的DNA靶的存在下。微阵列是通过将MB官能化的3 μ m直径微球随机分布在蚀刻在500 μ m直径光学成像纤维中的威尔斯阵列中来制备的。使用几个MB，每个设计识别不同的目标，我们证明了基因组囊性纤维化相关的目标的选择性检测。使用光学编码方案和成像荧光显微镜系统进行微球的位置配准和荧光响应监测。
We have developed a randomly ordered fiber-optic gene array for rapid, parallel detection of unlabeled DNA targets with surface immobilized molecular beacons (MB) that undergo a conformational change accompanied by a fluorescence change in the presence of a complementary DNA target. Microarrays are prepared by randomly distributing MB-functionalized 3-mu m diameter microspheres in an array of wells etched in a 500-mu m diameter optical imaging fiber. Using several MBs, each designed to recognize a different target, we demonstrate the selective detection of genomic cystic fibrosis related targets. Positional registration and fluorescence response monitoring of the microspheres was performed using an optical encoding scheme and an imaging fluorescence microscope system.