A Standardized Method for Detection of HLA-A and HLA-B Alleles by One-Dimensional Isoelectric Focusing (IEF) Gel Electrophoresis
A Standardized Method for Detection of HLA-A and HLA-B Alleles by One-Dimensional Isoelectric Focusing (IEF) Gel Electrophoresis
复制标题
一维等电聚焦 (IEF) 凝胶电泳检测 HLA-A 和 HLA-B 等位基因的标准化方法
DOI：
10.1007/978-1-4612-3552-1_66
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发表时间：
1989
影响因子：
3.9
通讯作者：
S. Yang
中科院分区：
文献类型：
--
作者：
S. Yang
采用一维等电聚焦(1D-IEF)凝胶电泳法对工作坊I类人类白细胞抗原生物化学部分中的I类分子进行了研究。所使用的方法是由O‘Farrell(1)最初开发的二维凝胶电泳法的改进版本。该工作坊技术利用了垂直平板凝胶仪和IEF样品，这些样品是通过使用单抗进行免疫沉淀从代谢标记的细胞裂解物中获得的。神经氨酸酶处理需要免疫沉淀的人类白细胞抗原，以获得每个抗原单一的、强表达的IEF条带。
One-dimensional isoelectric focusing (1D-IEF) gel electrophoresis was used for the study of class I HLA molecules in the Class I HLA Biochemistry component of the Workshop. The method used is a modified version of the two-dimensional gel electrophoresis technique originally developed by O’Farrell (1). The Workshop technique utilized a vertical slab gel apparatus and IEF samples obtained from metabolically labeled cell lysates by immunoprecipitation using monoclonal antibodies. The immunoprecipitated HLA antigens are desialated by neuraminidase treatment to obtain a single, strongly expressed IEF band for each antigen.