Critical roles for a genetic code alteration in the evolution of the genus Candida
Critical roles for a genetic code alteration in the evolution of the genus Candida
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DOI：
10.1038/sj.emboj.7601876
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发表时间：
2007-10-31
期刊：
影响因子：
11.4
通讯作者：
Santos, Manuel As
中科院分区：
文献类型：
--
作者：
Silva, Raquel M.;Paredes, Joao A.;Santos, Manuel As
在过去的 30 年里，在各种细菌和真核生物物种中发现了标准遗传密码的一些改变。有义和无义密码子已被重新分配或重新编程，以将遗传密码扩展到硒代半胱氨酸和吡咯赖氨酸。这些发现凸显了遗传密码中意想不到的灵活性，但没有阐明生物体如何在密码子身份重新定义产生的蛋白质组混乱中幸存下来。为了对这个问题提供新的认识，我们重建了酿酒酵母中念珠菌遗传密码的改变，并结合使用 DNA 微阵列、蛋白质组学和遗传学方法来评估其对基因表达、适应和有性生殖的影响。这种基因操作阻止了交配，将酵母锁定在二倍体状态，重塑了基因表达并产生了应激交叉保护，从而在环境挑战条件下产生了适应性优势。这项研究强调了念珠菌属进化过程中密码子身份重新定义的意想不到的作用，并强烈表明遗传密码的改变会产生加速物种形成的遗传障碍。
During the last 30 years, several alterations to the standard genetic code have been discovered in various bacterial and eukaryotic species. Sense and nonsense codons have been reassigned or reprogrammed to expand the genetic code to selenocysteine and pyrrolysine. These discoveries highlight unexpected flexibility in the genetic code, but do not elucidate how the organisms survived the proteome chaos generated by codon identity redefinition. In order to shed new light on this question, we have reconstructed a Candida genetic code alteration in Saccharomyces cerevisiae and used a combination of DNA microarrays, proteomics and genetics approaches to evaluate its impact on gene expression, adaptation and sexual reproduction. This genetic manipulation blocked mating, locked yeast in a diploid state, remodelled gene expression and created stress cross-protection that generated adaptive advantages under environmental challenging conditions. This study highlights unanticipated roles for codon identity redefinition during the evolution of the genus Candida, and strongly suggests that genetic code alterations create genetic barriers that speed up speciation.