Effects of ethanol extract of propolis on histopathological changes and anti-oxidant defense of kidney in a rat model for type 1 diabetes mellitus.
Effects of ethanol extract of propolis on histopathological changes and anti-oxidant defense of kidney in a rat model for type 1 diabetes mellitus.
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DOI：
10.1111/jdi.12459
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发表时间：
2016-07
影响因子：
3.2
通讯作者：
Safari M
中科院分区：
文献类型：
--
作者：
Sameni HR;Ramhormozi P;Bandegi AR;Taherian AA;Mirmohammadkhani M;Safari M
氧化应激在糖尿病的发病机制中起关键作用。蜂胶及其成分具有抗氧化应激的广泛药用特性。在本研究中，我们研究了蜂胶乙醇提取物对糖尿病大鼠肾脏的抗氧化作用。选取雄性Wistar大鼠40只，随机分为5组：对照组、糖尿病组、载药组、蜂胶组（100 mg/kg）和糖尿病组（200 mg/kg）。腹腔注射链脲佐菌素（60 mg/kg）诱导大鼠糖尿病。糖尿病组分别给予伊朗蜂胶载体提取物或乙醇提取物治疗6周。测定血清丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶的浓度。结果表明，伊朗蜂胶能显著抑制糖尿病大鼠的体重下降。蜂胶提取物显著降低糖尿病大鼠血清葡萄糖水平和肾脏重量（P < 0.001）。蜂胶提取物显著降低了糖尿病大鼠肾脏组织丙二醛含量，提高了超氧化物歧化酶和谷胱甘肽过氧化物酶活性（P < 0.001）和总抗氧化活性。糖尿病组和载药组肾脏肾小球基底膜厚度和肾小球面积均显著增加。蜂胶提取物对糖尿病大鼠肾小球基底膜厚度及肾小球面积均有显著降低。本研究结果表明，伊朗蜂胶提取物可以提高大鼠肾脏的抗氧化水平和组织病理学改变。最终结果表明，蜂胶的大部分有利作用是通过降低糖尿病动物的血糖水平介导的。
Oxidative stress has a key role in the pathogenesis of diabetes. Propolis and its constituents have a wide range of medicinal properties against oxidative stress. In the present study, we evaluated the anti‐oxidant effects of ethanolic extracts of propolis on kidneys in diabetes mellitus rats. A total of 40 male Wistar rats were randomly divided into the following five groups: control, diabetes mellitus, diabetes mellitus with vehicle treatment, diabetes mellitus with propolis treatment (100 mg/kg) and diabetes mellitus with propolis treatment (200 mg/kg). Diabetes mellitus in rats was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Diabetic groups were treated with vehicle or ethanolic extracts of Iranian propolis for 6 weeks. Serum concentration of malondialdehyde, superoxide dismutase and glutathione peroxidase were measured. The results showed that Iranian propolis significantly inhibited bodyweight loss in diabetes mellitus rats. The propolis extracts significantly reduced serum glucose levels and kidney weight in diabetes mellitus rats (P < 0.001). Furthermore, propolis extracts significantly reduced the malondialdehyde content, and increased the activity of superoxide dismutase and glutathione peroxidase (P < 0.001) along with the total anti‐oxidant activity in the kidney tissue of diabetes mellitus rats. In the kidneys of the diabetes mellitus and vehicle group, the glomerular basement membrane thickness and glomerular area were significantly increased. Treatment of diabetes mellitus rats with the propolis extract significantly reduced the glomerular basement membrane thickness and glomerular area. The present study results showed that the Iranian propolis extract could enhance the anti‐oxidant levels and histopathological changes in the kidneys of rats. The final results showed that most of the favorable effects of propolis are mediated by a reduction of blood glucose levels in diabetic animals.