Nitric oxide synthesis in patients with infective gastroenteritis
Nitric oxide synthesis in patients with infective gastroenteritis
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DOI：
10.1136/gut.45.3.355
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发表时间：
1999-09-01
期刊：
影响因子：
24.5
通讯作者：
Benjamin, N
中科院分区：
文献类型：
--
作者：
Forte, P;Dykhuizen, RS;Benjamin, N
背景：有证据表明，感染性胃肠炎患者的内源性硝酸盐合成明显增加。目的：确定这是否由于通过l -精氨酸/NO途径产生一氧化氮（NO）。方法：7例男性社区获得性细菌性胃肠炎患者和15例健康男性志愿者，均为粪便培养阳性的感染性胃肠炎患者。禁食过夜后静脉注射200 mg L-[N-15](2)-精氨酸。在接下来的36小时内收集尿液。用同位素比值质谱仪干燃烧法测定尿[N-15:N-14]硝酸盐比值。结果：胃肠炎组平均36小时尿硝态氮总排泄量为5157 (577)mu mol，对照组为2594 (234)mu mol
Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p