Lachesin: an immunoglobulin superfamily protein whose expression correlates with neurogenesis in grasshopper embryos.
Lachesin: an immunoglobulin superfamily protein whose expression correlates with neurogenesis in grasshopper embryos.
复制标题
DOI：
--
复制
发表时间：
1993-06
期刊：
影响因子：
4.6
通讯作者：
R. Karlstrom;Leigh P. Wilder;M. Bastiani
中科院分区：
文献类型：
--
作者：
R. Karlstrom;Leigh P. Wilder;M. Bastiani
我们描述了一种新的免疫球蛋白超家族蛋白Lachesin在蝗虫（美洲血吸虫）中的发育表达和在蝗虫和果蝇（果蝇）中的分子特征。Lachesin在中枢和外周神经系统中从神经发生开始在分化的神经元细胞的表面上表达。Lachesin的表达开始于神经源性外胚层的一些细胞中，紧接着在每个未来节段的后部细胞中开始进行表达。所有神经源性细胞早期表达Lachesin，但只有那些成为神经母细胞的细胞继续表达Lachesin。神经源性区域中的外胚层细胞在它们从潜在的神经源性途径偏离时失去Lachesin，所述外胚层细胞采用非神经元命运。成神经细胞、神经节母细胞和神经元在其生命早期都表达Lachesin，但随着发育的进展，表达变得限于神经元的子集。感觉神经元在从体壁外胚层分层时表达Lachesin。Lachesin也存在于CNS和PNS的生长轴突上，并且在发育后期变得限于轴突的子集。这种表达在已知的昆虫神经基因中是独特的，并表明Lachesin在早期神经元分化和轴突生长中的作用。蚱蜢Lachesin是一种通过糖基磷脂酰肌醇锚与细胞膜连接的38 × 10（3）M（r）蛋白。我们已经从蚱蜢和苍蝇中克隆了Lachesin基因。这两个物种之间的蛋白质是高度保守的（70%相同）。Lachesin类似于果蝇汞合金、牛OBCAM和人脊髓灰质炎病毒受体，将其置于含有一个V型和两个C2型免疫球蛋白结构域的免疫球蛋白超家族的亚组中。Lachesin也类似于已知在神经突生长和其他细胞表面识别事件中起作用的几种其他脊椎动物免疫球蛋白超家族蛋白（TAG-1、F11、L1和NgCAM）。
We describe the developmental expression in grasshopper (Schistocerca americana) and molecular characterization in grasshopper and fruit fly (Drosophila melanogaster) of Lachesin, a novel immunoglobulin superfamily protein. Lachesin is expressed on the surfaces of differentiating neuronal cells from the onset of neurogenesis in both the central and peripheral nervous systems. Lachesin expression begins in some cells of the neurogenic ectoderm immediately after engrailed expression begins in the posterior cells of each future segment. All neurogenic cells express Lachesin early, but only those cells that become neuroblasts continue to express Lachesin. Ectodermal cells in the neurogenic region that adopt non-neuronal fates lose Lachesin at the time that they diverge from a potentially neurogenic pathway. Neuroblasts, ganglion mother cells and neurons all express Lachesin early in their lives, but expression becomes restricted to a subset of neurons as development progresses. Sensory neurons express Lachesin as they delaminate from the body wall ectoderm. Lachesin is also present on growing axons of the CNS and PNS and becomes restricted to a subset of axons later in development. This expression is unique among known insect neurogenic genes and suggests a role for Lachesin in early neuronal differentiation and axon outgrowth. Grasshopper Lachesin is a 38 x 10(3) M(r) protein linked to cell membranes through a glycosyl phosphatidylinositol anchor. We have cloned the Lachesin gene from both grasshopper and fly. The proteins are highly conserved (70% identical) between the two species. Lachesin is similar to Drosophila amalgam, bovine OBCAM and the human poliovirus receptor, putting it into a subgroup of the immunoglobulin superfamily containing one V- and two C2-type immunoglobulin domains. Lachesin is also similar to several other vertebrate immunoglobulin superfamily proteins (TAG-1, F11, L1 and NgCAM) known to function in neurite outgrowth and other cell surface recognition events.