Orientation of smooth muscle-derived A10 cells in culture by cyclic stretching: Relationship between stress fiber rearrangement and cell reorientation
Orientation of smooth muscle-derived A10 cells in culture by cyclic stretching: Relationship between stress fiber rearrangement and cell reorientation
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DOI：
10.2108/zsj.15.617
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发表时间：
2000-07-01
影响因子：
0.9
通讯作者：
Obinata, T
中科院分区：
文献类型：
--
作者：
Hayakawa, K;Hosokawa, A;Obinata, T
机械应力在体内和体外引起细胞的各种反应。细胞和应力纤维的重新排列是应力引起的显著现象之一。然而，控制它们重新排列的机制在很大程度上是未知的。在这项研究中，机械拉伸对培养的细胞形态的影响进行了研究，使用循环和往复细胞拉伸装置。A10细胞，一种来自大鼠主动脉平滑肌的细胞系，被用作模型，因为它们是纺锤形的，并且具有显著的应力纤维，其沿纵向细胞轴沿着。因此，细胞和应力纤维的取向可以很容易地识别。当细胞在弹性硅酮膜上培养，并以每分钟60个循环的频率以20%的振幅进行循环和往复拉伸时，在拉伸开始后约15分钟内，肌动蛋白应力纤维以50至70度的角度倾斜于拉伸方向排列。然后，经过1-3小时的循环拉伸，大部分细胞的长轴也重新定向到与应力纤维相似的方向。拉伸诱导的细胞重定向被1 μ M细胞松弛素B阻断，但不被秋水仙胺阻断。这些结果表明，细胞的定向与肌动蛋白丝密切相关，肌动蛋白丝在细胞重定向的早期阶段起着至关重要的作用。
Mechanical stress causes various responses in cells both in vivo and in vitro. Realignment of cells and stress fibers is one of the remarkable phenomena that are induced by the stress. However, the mechanism by which their realignment is controlled is largely unknown. In this study, effects of mechanical stretch on the morphology of cultured cells were examined using a cyclic and reciprocal cell stretching apparatus. A10 cells, a cell line derived from rat aortic smooth muscle, were used as a model, since they are spindle-shaped and have remarkable stress fibers aligned along the longitudinal cell axis. Therefore, the orientation of the cell and stress fibers could be easily identified. When the cells were cultured on elastic silicone membranes and subjected to cyclic and reciprocal stretch with an amplitude of 20% at a frequency of 60 cycles per minute, actin stress fibers were aligned obliquely to the direction of stretching with angles of 50 to 70 degrees within about 15 min after the onset of stretching. Then, after 1-3 hr of cyclic stretching, the long axes of a majority of the cells were also reoriented to similar directions to the stress fibers. The stretch-induced cell reorientation was blocked by 1 mu M cytochalasin B, but not by colcemid. These results indicate that the orientation of cells and actin filaments are closely related and actin filaments play a critical role in the early step of the cell reorientation.