FORMATION OF WATER-SOLUBLE MONASCUS-RED PIGMENTS BY BIOLOGICAL AND SEMISYNTHETIC PROCESSES
FORMATION OF WATER-SOLUBLE MONASCUS-RED PIGMENTS BY BIOLOGICAL AND SEMISYNTHETIC PROCESSES
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DOI：
10.1007/bf01569621
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发表时间：
1992-05-01
期刊：
影响因子：
--
通讯作者：
DEMAIN, AL
中科院分区：
文献类型：
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作者：
LIN, TF;YAKUSHIJIN, K;DEMAIN, AL
在以谷氨酸为氮源的化学成分确定的发酵培养基中，由单胞菌产生新的水溶性红色素。他们被分离和其特征在于众所周知的橙子Monascoruridine色素（monascoruridine和rubropunctatin）的谷氨酸衍生物。与已知的红色Monoclonal颜料（红点红胺和monascorubramine）相比，新颜料具有几个优点，包括非常高的水溶性、更高的吸收系数和更大的耐光脱色性。将谷氨酸、甘氨酸或亮氨酸添加到静止细胞系统中，导致与外源氨基酸对应的特定水溶性红色素的形成。由静止细胞产生的水溶性红色素的保留时间与由橙子色素在pH 7的甲醇-磷酸盐缓冲液中化学制备的相应红色衍生物的保留时间相同。氨基酸源的疏水性对应于由其衍生的红色颜料的HPLC保留时间，
New water-soluble red pigments were produced by Monascus sp. in a chemically defined fermentation medium containing glutamate as nitrogen source. They were isolated and characterized as glutamate derivatives of the well-known orange Monascus pigments (monascorubrin and rubropunctatin). The new pigments have several advantages over the known red Monascus pigments (rubropunctamine and monascorubramine) including very high water-solubility, higher absorption coefficient, and greater resistance to decoloration by light. Adding glutamate, glycine or leucine to a resting-cell system led to the formation of specific water-soluble red pigments corresponding to the exogenous amino acid. The water-soluble red pigments produced by resting-cells have retention times identical to those of the corresponding red derivatives made chemically from the orange pigments in methanol-phosphate buffer at pH 7. The hydrophobicities of the amino acid sources correspond to the HPLC retention times of the red pigments derived from them,