Purification and characterization of class-I and class-II fructose-1,6-bisphosphate aldolases from the cyanobacterium Synechocystis sp PCC 6803
Purification and characterization of class-I and class-II fructose-1,6-bisphosphate aldolases from the cyanobacterium Synechocystis sp PCC 6803
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DOI：
10.1093/pcp/pcg044
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发表时间：
2003-03-01
影响因子：
4.9
通讯作者：
Yokota, A
中科院分区：
文献类型：
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作者：
Nakahara, K;Yamamoto, H;Yokota, A
集胞藻 (Synechocystis sp) 的全基因组序列数据库。 PCC 6803 揭示了该生物体中存在编码 I 类 (CI) 和 II 类 (CII) 果糖-1,6-二磷酸醛缩酶 (FBA) 的基因。来自集胞藻属的两种 FBA。 PCC 6803 通过苯基琼脂糖色谱法分离。主峰中酶的活性因 25 mM EDTA 的存在而受到抑制；然而，小高峰时期的活动却并非如此。因此，前一个级分中的FBA被指定为CII-FBA，而后者中的FBA被指定为CI-FBA。 CI-FBA 在集胞藻中具有功能冗余。 PCC 6803，而在光自养条件下没有获得编码 CII-FBA 的基因的破坏。从集胞藻中纯化的 CI-和 CII-FBA 的动力学参数。 PCC 6803 在 FBP 裂解反应中大体相似，除了它们对 SBP 的反应性不同。 CII-FBA 的 SBP/FBP 活性比是 CI-FBA 的两倍。
The whole genome sequence database for Synechocystis sp. PCC 6803 has revealed the presence of genes encoding class-I (CI) and class-II (CII) fructose-1,6-bisphosphate aldolases (FBAs) in this organism. Two types of FBA from Synechocystis sp. PCC 6803 were separated by chromatography on phenyl-Sepharose. The activity of the enzyme in the major peak was inhibited by the presence of 25 mM EDTA; however, the activity in the minor peak was not. Therefore, the FBA in the former fractions was designated as CII-FBA, and in the latter designated as CI-FBA. CI-FBA was functionally redundant in Synechocystis sp. PCC 6803, while no disruptant for the gene encoding CII-FBA was obtained under photoautotrophic conditions. The kinetic parameters of CI- and CII-FBAs purified from Synechocystis sp. PCC 6803 in the cleavage reaction of FBP were generally similar, except in their reactivity for SBP. The SBP/FBP activity ratio of the CII-FBA was two times higher than that of the CI-FBA.