Evidence of random structural features in the heparin polymer.
Evidence of random structural features in the heparin polymer.
复制标题
肝素聚合物随机结构特征的证据。
DOI：
10.1021/bi00347a045
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Langer,R
中科院分区：
文献类型：
--
作者：
Linhardt,RJ;Merchant,ZM;Rice,KG;Kim,YS;Fitzgerald,GL;Grant,AC;Langer,R
据报道，首次使用计算机模拟研究来检查肝素的结构。用肝素酶解聚猪粘膜肝素得到的产物分布与计算机模拟的分布进行了比较。模型分布相对不受肝素的多分散性和分子量的影响。然而，肝素酶可切割的糖苷键的百分比及其在聚合物中的分布导致模拟产物分布的显着变化。实验观察和计算机模拟产物分布的相似性与猪粘膜肝素中肝素酶可切割位点的随机分布是一致的。最后，实验观察到n -乙酰基残基相对于肝素酶可切割位点的随机分布。
The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydispersity and molecular weight of heparin. However, the percent of heparinase-cleavable glycosidic linkages and their distribution throughout the polymer resulted in a marked change in the simulated product distribution. The similarity between experimentally observed and computer-simulated product distributions is consistent with the random distribution of heparinase-cleavable sites in porcine mucosal heparin. Finally, a random distribution of N-acetyl residues with respect to heparinase-cleavable sites was experimentally observed.