Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata
Transcriptomic responses to heat stress and bleaching in the elkhorn coral Acropora palmata
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DOI：
10.3354/meps08372
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Medina, Monica
中科院分区：
文献类型：
--
作者：
DeSalvo, Michael K.;Sunagawa, Shinichi;Medina, Monica
用于造礁珊瑚和共生海葵的基因组工具的出现正值全球珊瑚覆盖面积出现令人震惊的损失之际。这些工具具有很大的希望，在阐明新的和不可预见的细胞过程的基础上成功的珊瑚和他们的甲藻内共生体共生藻属之间的互惠关系。由于热应力触发共生（珊瑚漂白）的故障，测量转录组响应热应力诱导漂白提供了一个非凡的看法，是特定于珊瑚藻共生的细胞过程。在本研究中，我们利用一个cDNA微阵列含有2059个基因的受威胁的加勒比海埃尔克霍恩珊瑚鹿角掌形珊瑚，以确定差异表达的基因热应力。将来自重复菌落的片段暴露于高温2 d，并在24和48 h后冷冻样品用于微阵列分析。在热应激后1 d和2 d采集的样品中，分别有204个和104个基因差异表达。差异表达基因的分析表明A.掌状体的功能包括（1）生长停滞，（2）伴侣活性，（3）核酸稳定和修复，以及（4）去除受损的大分子。其他差异表达的过程包括感觉知觉，宿主和内共生体之间的代谢物转移，一氧化氮信号传导，以及肌动蛋白细胞骨架和细胞外基质的修饰。结果进行了比较，从以前的珊瑚微阵列研究Montastraea faveolata的热应力，并指出整体进化保守的漂白反应在石珊瑚。
The emergence of genomic tools for reef-building corals and symbiotic anemones comes at a time when alarming losses in coral cover are being observed worldwide. These tools hold great promise in elucidating novel and unforeseen cellular processes underlying the successful mutualism between corals and their dinoflagellate endosymbionts Symbiodinium spp. Since thermal stress triggers a breakdown in the symbiosis (coral bleaching), measuring the transcriptomic response to thermal stress-induced bleaching offers an extraordinary view of cellular processes that are specific to coral algal symbioses. In the present study, we utilized a cDNA microarray containing 2059 genes of the threatened Caribbean elkhorn coral Acropora palmata to identify genes that are differentially expressed upon thermal stress. Fragments from replicate colonies were exposed to elevated temperature for 2 d, and samples were frozen for microarray analysis after 24 and 48 h. Totals of 204 and 104 genes were differentially expressed in samples that were collected 1 and 2 d after thermal stress, respectively. Analysis of the differentially expressed genes indicates a cellular stress response in A. palmata involving (1) growth arrest, (2) chaperone activity, (3) nucleic acid stabilization and repair, and (4) removal of damaged macromolecules. Other differentially expressed processes include sensory perception, metabolite transfer between host and endosymbiont, nitric oxide signaling, and modifications to the actin cytoskeleton and extracellular matrix. The results are compared with those from a previous coral microarray study of thermal stress in Montastraea faveolata, and point to an overall evolutionary conserved bleaching response in scleractinian corals.