Early onset of phenotype and cell patterning in the embryonic zebrafish retina.
Early onset of phenotype and cell patterning in the embryonic zebrafish retina.
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DOI：
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发表时间：
1990-07
期刊：
影响因子：
4.6
通讯作者：
K. Larison;R. Bremiller
中科院分区：
文献类型：
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作者：
K. Larison;R. Bremiller
视网膜锥体细胞排列规则，呈马赛克状，是硬骨鱼的常见特征。在斑马鱼Brachydanio rerio中，视网膜锥体马赛克由四种锥体类型的重复主题组成的平行排列组成。为了阐明发育中视网膜中视锥花叶发生的时间和空间方面，我们制备了一种能与成人双视锥感光细胞特异结合的单抗。我们在受精后48小时首次看到这种抗体FRET 43在发育中的视网膜中染色，这是第一批光感受器细胞进行最后有丝分裂的时候。然后我们给胚胎鱼注射胸苷类似物5-溴-2‘-脱氧尿苷(BrdU)，用双标记实验证实FRET 43抗原性的开始发生在产生双锥体光感受器的细胞分裂的三个小时内。然后，我们用FRET 43对54小时胚胎视网膜的切面进行了染色，进一步表明，没有染色的细胞与染色的细胞对以一种与成年视锥马赛克中光感受器的排列一致的模式交替。这些结果表明，双锥体表型的一个标记与细胞生日几乎同时表达，并且在表达后6小时内出现马赛克图案。
The regular arrangement of retinal cone cells in a mosaic pattern is a common feature of teleosts. In the zebrafish, Brachydanio rerio, the retinal cone mosaic comprises parallel rows consisting of a repeating motif of four cone types. In order to elucidate the temporal and spatial aspects of the genesis of the cone mosaic in the developing retina, we generated a monoclonal antibody that specifically binds to the double cone photoreceptor of the adult. We first saw staining in the developing retina with this antibody, FRet 43, at 48 hours postfertilization, the time at which the first photoreceptor cells undergo their final mitotic division. We then injected embryonic fish with the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU), confirming with a double-labeling experiment that the onset of FRet 43 antigenicity occurs within three hours of the cellular division that generates the double cone photoreceptors. Then we stained tangential sections of the 54-hour embryonic retina with FRet 43, further showing that cells devoid of staining alternate with stained pairs of cells in a pattern that is consistent with the arrangement of photoreceptors in the adult cone mosaic. These results indicate that a marker of the double cone phenotype is expressed at approximately the same time as cellular birthday and that the mosaic patterning is present within 6 hours of this expression.