Co-localization of non-histone protein BA with U-snRNPs to the same regions of the cell nucleus.
Co-localization of non-histone protein BA with U-snRNPs to the same regions of the cell nucleus.
复制标题
非组蛋白 BA 与 U-snRNP 共定位于细胞核的相同区域。
DOI：
10.1016/0014-4827(85)90123-5
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Yeoman,LC
中科院分区：
文献类型：
--
作者：
Bennett,FC;Yeoman,LC
应用间接免疫荧光技术，将核非组蛋白BA定位于正常大鼠肝细胞系。蛋白BA抗体免疫染色核结构，形成斑点状免疫荧光染色图案。BA蛋白抗体染色的细胞核结构对DNase I消化敏感，但对RNase不敏感。用蛋白BA抗体观察到的核荧光斑点图案与早先报道的与U-SnRNPs反应的Sm抗体相似。用双标记间接免疫荧光显示，Sm抗原集中在含有BA蛋白的细胞核中。用这两种抗体对总核蛋白的免疫印迹分析表明，BA蛋白和主要的Sm抗原具有相似的分子量，但在抗原性上是不同的。此外，它们从细胞核中提取的能力也不同。
Using indirect immunofluorescence, nuclear non-histone protein BA was localized in a normal rat liver cell line. Protein BA antibodies immunostained nuclear structures producing a speckled immunofluorescent staining pattern. Nuclear structures stained with protein BA antibodies were sensitive to DNase I digestion, but not to RNase. The speckled pattern of nuclear fluorescence observed with protein BA antibodies was similar to that reported earlier for Sm antibodies, which react with U-snRNPs. Using double-label indirect immunofluorescence, the Sm antigen was shown to be concentrated in the same regions of the nucleus which contain protein BA. Immunoblot analysis of total nuclear proteins with the two antibodies demonstrated that protein BA and the major Sm antigen have similar molecular weights, but are antigenically distinct. In addition, they differ in their extractabilities from the cell nucleus.