Human Germinal Center-associated Lymphoma (HGAL) Is a Reliable Marker of Normal and Neoplastic Follicular Helper T Cells Including Angioimmunoblastic T-Cell Lymphoma.
Human Germinal Center-associated Lymphoma (HGAL) Is a Reliable Marker of Normal and Neoplastic Follicular Helper T Cells Including Angioimmunoblastic T-Cell Lymphoma.
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人类生发中心相关淋巴瘤 (HGAL) 是正常和肿瘤性滤泡辅助 T 细胞（包括血管免疫母细胞 T 细胞淋巴瘤）的可靠标志物。
DOI：
10.1097/pas.0000000000001852
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Natkunam,Yasodha
中科院分区：
文献类型：
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作者：
Koo,Matthew;Zhang,Jingjing;Tan,Brent;Kurzer,Jason;Gratzinger,Dita;Zhao,Shuchun;Suarez,Carlos;Lossos,IzidoreS;Warnke,RogerA;Natkunam,Yasodha
血管免疫母细胞性T细胞淋巴瘤(AITL)的诊断很复杂，需要T滤泡助手(TFH)表型的证明。检测TFH表型的免疫表型标记物是高度可变的，因此有必要使用3到5个TFH标记物来证实TFH表型。我们测试了生发中心标记物人类生发中心相关淋巴瘤(HGAL)和LIM结构域2(LMO2)在检测TFH表型中的作用。我们将它们的染色与目前使用的6个TFH标记物PD-1、ICOS、CXCL13、SAP、CD10和BCL6在23个AITL队列中的染色进行了比较。我们的结果表明，虽然两种标记都可以检测到TFH表型，但HGAL在细胞染色百分比和染色强度方面优于LMO2，这两个变量用于产生H-分数。以H评分为标准，在目前使用的TFH标志物中，HGAL与BCL6最相似，比CXCL13、SAP、CD10和LMO2更敏感。在这项研究中测试的8个标记中，PD-1和ICOS在检测TFH表型方面是最可靠的。我们认为，HGAL是TFH细胞的可靠标记物，有助于TFH来源的淋巴瘤的诊断，特别是在识别AITL的早期模式方面。
The diagnosis of angioimmunoblastic T-cell lymphoma (AITL) is complex and requires the demonstration of a T-follicular helper (TFH) phenotype. Immunophenotypic markers that detect the TFH phenotype are highly variable, thereby necessitating the use of 3 to 5 TFH markers to substantiate a TFH phenotype. We tested the utility of germinal center markers human germinal center–associated lymphoma (HGAL) and LIM-domain only 2 (LMO2) in detecting a TFH phenotype. We compared their staining to that of 6 TFH markers in current use, PD-1, ICOS, CXCL13, SAP, CD10, and BCL6, in a cohort of 23 AITL. Our results show that although both markers can detect a TFH phenotype, HGAL was superior to LMO2 in the percent of cells stained and the intensity of staining, 2 variables used to generate H-scores. Using H-scores as the metric, HGAL was most comparable to BCL6 among the currently used TFH markers and was more sensitive than CXCL13, SAP, CD10, and LMO2. PD-1 and ICOS emerged as the most robust of the 8 markers tested in this study in detecting a TFH phenotype. We conclude that HGAL is a reliable marker of TFH cells and can aid in the diagnosis of lymphomas of TFH derivation, particularly in the recognition of early patterns of AITL.