A SNF2-like protein facilitates dynamic control of DNA methylation
A SNF2-like protein facilitates dynamic control of DNA methylation
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DOI：
10.1038/sj.embor.7400446
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发表时间：
2005-07-01
期刊：
影响因子：
7.7
通讯作者：
Matzke, AJM
中科院分区：
文献类型：
--
作者：
Kanno, T;Aufsatz, W;Matzke, AJM
DRD1是一种SNF2样蛋白，以前在筛选拟南芥种子启动子RNA指导的DNA甲基化缺陷突变体中鉴定出。尽管最初的研究确定了DRD1在RNA指导的DNA甲基化中的作用，但它并没有解决DRD1是否需要从头或维持甲基化，或者它是否需要其他靶序列的甲基化。我们在这里表明，DRD1是必不可少的RNA指导的从头甲基化和作用于不同的目标启动子。此外，当研究分离沉默触发物后的维持甲基化时，显示了DRD1在CG甲基化消除中的意外作用。DRD1在已知的SNF2样蛋白中是独特的，不仅促进靶序列响应于RNA信号的从头甲基化，而且当沉默诱导物被撤回时甲基化的丧失。DRD1的相反作用可能有助于DNA甲基化的动态调节。
DRD1 is a SNF2-like protein previously identified in a screen for mutants defective in RNA-directed DNA methylation of a seed promoter in Arabidopsis. Although the initial study established a role for DRD1 in RNA-directed DNA methylation, it did not address whether DRD1 is needed for de novo or maintenance methylation, or whether it is required for methylation of other target sequences. We show here that DRD1 is essential for RNA-directed de novo methylation and acts on different target promoters. In addition, an unanticipated role for DRD1 in erasure of CG methylation was shown when investigating maintenance methylation after segregating away the silencing trigger. DRD1 is unique among known SNF2-like proteins in facilitating not only de novo methylation of target sequences in response to RNA signals, but also loss of methylation when the silencing inducer is withdrawn. The opposing roles of DRD1 could contribute to the dynamic regulation of DNA methylation.