Solvatochromism of the green fluorescence protein chromophore and its derivatives
Solvatochromism of the green fluorescence protein chromophore and its derivatives
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DOI：
10.1021/ja063128a
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发表时间：
2006-09-20
影响因子：
15
通讯作者：
Tolbert, Laren M.
中科院分区：
文献类型：
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作者：
Dong, Jian;Solntsev, Kyril M.;Tolbert, Laren M.
利用紫外-维斯吸收光谱研究了绿色荧光蛋白（GFP）发色团（p-hydroxybenzylideneimidazolone，p-HBDI）及其衍生物（p-methoxybenzylideneimidazolone，p-MeOBDI和N-甲基-p-hydroxybenzylideneimidazoloniumiodide，p-HBDIMe+）在多种溶剂中的溶致变色行为。研究了特异性（极性）与非特异性（氢键）溶剂化对吸收光谱的相对贡献。在这些数据的基础上，我们讨论了野生型GFP的质子化和去质子化形式的吸收峰的性质。
The solvatochromic behavior of the green fluorescence protein (GFP) chromophore (p-hydroxybenzylideneimidazolone,p-HBDI) and its derivatives (p-methoxybenzylideneimidazolone,p-MeOBDI, andN-methyl-p-hydroxybenzylideneimidazolonium iodide,p-HBDIMe+) was studied using UV−vis−absorption spectroscopy in a wide array of solvents. The relative contribution of specific (polarity) vs nonspecific (hydrogen-bonding) solvation to the absorbance spectra was studied. On the basis of these data, we discuss the nature of the absorption peak of the protonated and deprotonated forms of the wild-type GFP.