Imprinted Genes and Satellite Loci Are Differentially Methylated in Bovine Somatic Cell Nuclear Transfer Clones
Imprinted Genes and Satellite Loci Are Differentially Methylated in Bovine Somatic Cell Nuclear Transfer Clones
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DOI：
10.1089/cell.2013.0012
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发表时间：
2013-10-01
影响因子：
1.6
通讯作者：
Chen, Chuan-Mu
中科院分区：
文献类型：
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作者：
Shen, Chih-Jie;Lin, Chiao-Chieh;Chen, Chuan-Mu
在哺乳动物中，全基因组表观遗传重编程系统存在于原始生殖细胞和合子中。这些重编程系统在胚胎发育的关键阶段调节基因组功能中起着至关重要的作用，并且它们赋予哺乳动物发育期间基因表达的稳定性。体细胞核移植（SCNT）后代的意外丢失是一个持续存在的问题。在这项研究中，我们使用了6个克隆牛（命名为NT-1至NT-6），这是通过耳成纤维细胞核移植创建的，并显示多器官缺陷的寿命短，作为实验模型。我们集中在三个印记基因（IGF 2，H19和XIST）和四个卫星位点（卫星I，卫星II，Art 2和VNTR），以研究其甲基化变化。结果表明，克隆牛的印迹基因常发生异常甲基化，而卫星基因座不发生异常甲基化。5-Aza-dc去甲基化剂处理牛成纤维细胞后，XIST和H19推定差异甲基化区域（DMR）的甲基化百分比显著降低（XIST，p
In mammals, genome-wide epigenetic reprogramming systems exist in primordial germ cells and zygotes. These reprogramming systems play crucial roles in regulating genome functions during critical stages of embryonic development, and they confer the stability of gene expression during mammalian development. The frequent unexpected loss of progeny from somatic cell nuclear transfer (SCNT) is an ongoing problem. In this study, we used six cloned bovines (named NT-1 to NT-6), which were created by ear fibroblast nuclear transfer and displayed short life spans with multiple organ defects, as an experimental model. We focus here on three imprinted genes (IGF2, H19, and XIST) and four satellite loci (Satellite I, Satellite II, Art2, and VNTR) to investigate their methylation changes. The results revealed that aberrant methylation frequently occurred in the analyzed imprinted genes, but not in the satellite loci, of the cloned bovines. After the bovine fibroblast cells were treated with the 5-aza-2()-deoxycytidine (5-Aza-dc) demethylation agent, the methylation percentages of the XIST and H19 putative differentially methylated region (DMR) were significantly decreased (XIST, p