TERATOGENIC EFFECTS OF EXCESS GLUCOSE ON HEAD-FOLD RAT EMBRYOS IN CULTURE
TERATOGENIC EFFECTS OF EXCESS GLUCOSE ON HEAD-FOLD RAT EMBRYOS IN CULTURE
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DOI：
10.1002/tera.1420160205
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
COPPOLA, PT
中科院分区：
文献类型：
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作者：
COCKROFT, DL;COPPOLA, PT
将妊娠9.5天（头折早期）的大鼠胚胎在含有大鼠血清和气相的旋转瓶中培养48小时。向培养基中添加12-15 mg/ml的D-葡萄糖导致严重异常，并在高比例的胚胎中延迟发育，无论添加葡萄糖所引起的渗透压增加是否得到纠正。渗透压升高增加了观察到的畸形的频率和严重程度。L-葡萄糖没有产生异常，但胚胎发育迟缓。
Rat embryos explanted at 9.5 days of gestation (early head-fold stages) were cultured for 48 h in rotating bottles containing rat serum and a gas phase. Addition of 12-15 mg/ml of D-glucose to the culture medium resulted in severe abnormalities and retarded development in a high proportion of embryos, whether or not the osmolarity increase brought about by the addition of glucose was corrected. The raised osmolarity increased the frequency and severity of malformations observed. L-glucose produced no abnormalities, though embryonic development was retarded.