Effect of intrahepatic omental implantation on angiogenesis in rat liver with hepatic artery ligation
Effect of intrahepatic omental implantation on angiogenesis in rat liver with hepatic artery ligation
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DOI：
10.1007/s10238-001-8006-3
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发表时间：
2001-03-01
影响因子：
4.6
通讯作者：
Tashiro, S
中科院分区：
文献类型：
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作者：
Ishikawa, M;Fjii, M;Tashiro, S
尽管进行了早期手术干预，治疗肝动脉血栓的效果仍然不佳。大网膜瓣的使用因其在促进缺血组织中的新血管形成方面的血管生成潜力而得到充分证明。该实验研究评估了大鼠肝动脉结扎后网膜植入（01）后新血管的形成。使用Wistar大鼠并将其分为以下组：1×01有HAL组、11×01无HAL组、III组肝动脉结扎(HAL)。对于血管造影，通过激光多普勒法测量肝组织血流量，通过彩色微球法（CMS）测量肝动脉血流。对血管内皮生长因子（VEGF）进行免疫组化研究。剖腹手术后1、3、7或30天处死大鼠。根据 CMS 测定，第 1 组植入叶中的相对动脉肝血流量在术后 7 天达到对照值的 50%。对切除肝脏的血管造影和显微镜研究显示，术后 7 天肝脏内网膜植入物周围有明显的血管生成。 01后在没有HAL的肝脏中没有观察到新血管的形成。大网膜植入似乎有助于预防肝动脉血栓形成后的器官缺氧。
Despite early surgical intervention, the results of treating hepatic artery thrombosis remain poor. The use of omental flaps is well documented for its angiogenic potential in promoting neovascularization in ischemic tissues. This experimental study evaluated the formation of new blood-vessels after omental implantation (01) in rats after ligation of the hepatic artery. Wistar rats were used and divided into the following groups: 1 01 with HAL, 11 01 without HAL, III hepatic artery ligation (HAL). For angiography, measurements were made of liver tissue blood flow by the laser Doppler method and of hepatic artery flow by the colored microsphere method (CMS). and immunohistochemical study was done for vascular endothelial growth factor (VEGF). The rats were killed at 1, 3, 7 or 30 days after laparotomy. Relative arterial hepatic blood flow in the implanted lobe of group 1, as determined by CMS, reached 50% of control values 7 days after surgery. Angiography and microscopic studies of the excised liver revealed distinct angiogenesis surrounding the omental implant in the liver for 7 days postoperatively. The formation of new blood-vessels after 01 was not observed in livers without HAL. Omental implantation appears to be useful in preventing organ anoxia after hepatic artery thrombosis.