The phylogenetic distribution of red cell 2,3 diphosphoglycerate and its interaction with mammalian hemoglobins.
The phylogenetic distribution of red cell 2,3 diphosphoglycerate and its interaction with mammalian hemoglobins.
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红细胞 2,3 二磷酸甘油酸的系统发育分布及其与哺乳动物血红蛋白的相互作用。
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
A. Brush
中科院分区：
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作者：
A. F. Scott;H. Bunn;A. Brush
为了更好地了解2，3-二磷酸甘油酸（DPG）在哺乳动物红细胞功能中的作用程度，我们测定了来自14个目的71个物种的血液中的DPG水平。此外，对于66个物种和4种血红蛋白表型的绵羊，DPG对氧亲和力的影响进行了测量，通过确定P50值的血红蛋白在DPG的情况下，在0.2 mM和1.0 mM的浓度。大多数哺乳动物有高水平的红细胞DPG和无磷血红蛋白与相对较高的氧亲和力。相反，两个分类学上不相关的组都有非常低的红细胞内DPG浓度以及天然低氧亲和力的血红蛋白与DPG弱相互作用。后一组包括Feloidea（食肉目）和Bovoidea（偶蹄目）。定量地处理DPG浓度、血红蛋白氧亲和力以及DPG与血红蛋白的相互作用之间的关系，以提供哺乳动物红细胞功能的模型。推导出的表达式与全血P50的描述性异速生长方程进行比较，并提供统计学上合理的预测。
In order to better understand the extent to which 2,3 diphosphoglycerate (DPG) contributes to red cell function in the mammals, we assayed DPG levels in blood from a taxonomically diverse set of 71 species representing 14 orders. In addition, for 66 species and 4 hemoglobin phenotypes of the sheep, the effect of DPG on oxygen affinity was measured by determining P 50 values for hemoglobin in the absence of DPG and at 0.2 mM and 1.0 mM concentrations. Most mammals had high levels of red cell DPG and phosphate-free hemoglobins with a relatively high oxygen affinity. In contrast, two taxonomically unrelated groups had both very low intra-erythrocytic DPG concentrations as well as hemoglobins of native low oxygen affinity that interacted weakly with DPG. This latter group includes the Feloidea (order Carnivora) and the Bovoidea (order Artiodactyla). The relationship between DPG concentration, hemoglobin oxygen affinity and the interaction of DPG with hemoglobin is treated quantitatively to provide a model of mammalian red cell function. This derived expression is compared with descriptive allometric equations for whole blood P 50 and is shown to provide statistically reasonable predictions.