Osmotic pressure inhibition of DNA ejection from phage
Osmotic pressure inhibition of DNA ejection from phage
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DOI：
10.1073/pnas.1233721100
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发表时间：
2003-08-05
影响因子：
11.1
通讯作者：
Gelbart, WM
中科院分区：
文献类型：
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作者：
Evilevitch, A;Lavelle, L;Gelbart, WM
水溶液中的细菌病毒衣壳可以通过加入它们的特异性受体蛋白在体外打开，从而完全排出它们的基因组。我们证明，它是可能的，以控制这种喷射的程度，通过改变外部渗透压。在λ噬菌体的特定情况下，喷射被（聚乙二醇的）渗透压抑制50%，与在宿主细菌细胞质中起作用的渗透压相当;它被20个大气压的压力完全抑制。此外，我们的实验直接监测的应力急剧下降后，未打开的噬菌体衣壳内的多价阳离子添加到主机解决方案，与最近的许多理论的DNA相互作用。
Bacterial viral capsids in aqueous solution can be opened in vitro by addition of their specific receptor proteins, with consequent full ejection of their genomes. We demonstrate that it is possible to control the extent of this ejection by varying the external osmotic pressure. In the particular case of bacteriophage lambda, the ejection is 50% inhibited by osmotic pressures (of polyethylene glycol) comparable to those operative in the cytoplasm of host bacteria; it is completely suppressed by a pressure of 20 atmospheres. Furthermore, our experiments monitor directly a dramatic decrease of the stress inside the unopened phage capsid upon addition of polyvalent cations to the host solution, in agreement with many recent theories of DNA interactions.