Transfer RNA mimicry among tymoviral genomic RNAs ranges from highly efficient to vestigial
Transfer RNA mimicry among tymoviral genomic RNAs ranges from highly efficient to vestigial
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DOI：
10.1093/nar/26.19.4356
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发表时间：
1998-10-01
影响因子：
14.9
通讯作者：
Goodwin, JB
中科院分区：
文献类型：
--
作者：
Dreher, TW;Goodwin, JB
已经使用高等植物（小麦胚芽）蛋白质定量评估了一组代表性的tymoviral RNA的三种tRNA相关性质：氨酰化，EF-1 α。GTP结合，以及通过CTP、ATP：tRNA核苷酸转移酶对RNA的3 '-CC形式的3'-腺苷酸化。RNA分为三类，不同的tRNA模仿的程度。芜菁黄花叶病毒（TYMV）和肯尼迪黄花叶病毒RNA在所有三个性质上都具有与高等植物tRNA（瓦尔）转录物相似的活性，因此是显著的tRNA模拟物。尽管分离的类似于83 nt长的tRNA样结构在这些测定中显示出高活性，但在TYMV的情况下，6318 nt长的TYMV RNA是缬氨酰化的甚至更好的底物，具有不同构建的受体茎假结的茄子花叶病毒RNA与上述的烟草花叶病毒RNA的不同之处在于与EF-1 α的结合更弱。GTP糖芥潜伏病毒RNA缺乏可识别的反密码子结构域，不能被缬氨酰化，并且具有非常低的3 '-腺苷酸化活性。因此，在tymovirus属内的tRNA模仿的范围从非常高度发达到最小，tRNA模仿在病毒生物学中的作用的影响进行了讨论。
Three tRNA-associated properties of a representative set of tymoviral RNAs have been quantitatively assessed using higher plant (wheat germ) proteins: aminoacylation, EF-1 alpha . GTP binding, and 3'-adenylation of 3'-CC forms of the RNAs by CTP, ATP:tRNA nucleotidyltransferase. The RNAs fall into three classes differing in the extent of tRNA mimicry. Turnip yellow mosaic (TYMV) and kennedya yellow mosaic virus RNAs had activities in all three properties similar to those of a higher plant tRNA(Val) transcript, and thus are remarkable tRNA mimics. Although the isolated similar to 83 nt long tRNA-like structures showed high activity in these assays, in the case of TYMV, the 6318 nt long TYMV RNA was an even better substrate for valylation, Eggplant mosaic virus RNA, which has a differently constructed acceptor stem pseudoknot, differed from the above tymoviral RNAs in binding more weakly to EF-l alpha . GTP. Erysimum latent virus RNA, which lacks an identifiable anticodon domain, could not be valylated and had very low 3'-adenylation activity. The range of tRNA mimicry within the tymovirus genus thus ranges from extremely highly developed to minimal, The implications on the role of the tRNA mimicry in viral biology are discussed.